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Sample GSM1903919 Query DataSets for GSM1903919
Status Public on Oct 10, 2015
Title Th2cell_RNA_exp1
Sample type SRA
 
Source name Five-day cultured Th2 cells generated from primary splenic CD4_T cells
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: Th2 cell
Growth protocol Splenic CD4_T cells were stimulated under Th2 culture conditions (15 ng/ml IL-2, 10 ng/ml IL-4) for 5 days in vitro.
Extracted molecule total RNA
Extraction protocol Total cellular RNA was extracted with TRIzol reagent (Invitrogen).
TruSeq RNA Sample Prep Kit v2 (Illumina) was used according to the manufacturer's protocol.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Description Th2_cells
Data processing Read sequences (50 bp) were aligned to the mm10 mouse reference genome (University of California, Santa Cruz (UCSC) December 2011) using Bowtie (version 0.12.8) and TopHat (version 1.3.2).
Fragments per kilobase of exon per million mapped reads (FPKM) for each gene were calculated using Cufflinks (version 2.0.2).
Genome_build: mm10
Supplementary_files_format_and_content: Text files include FPKM values for each Sample
 
Submission date Oct 07, 2015
Last update date May 15, 2019
Contact name Atsushi Onodera
Organization name Chiba University
Department Immunology
Street address Chuo-ku Inohana 1-8-1
City Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platform ID GPL18480
Series (2)
GSE73813 Spatial interplay between Polycomb and Trithorax complexes controls transcriptional activity in T lymphocytes [RNA-Seq]
GSE73825 Spatial interplay between Polycomb and Trithorax complexes controls transcriptional activity in T lymphocytes
Relations
BioSample SAMN04151785
SRA SRX1310208

Supplementary file Size Download File type/resource
GSM1903919_Th2_exp1.txt.gz 250.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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