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Sample GSM1903918 Query DataSets for GSM1903918
Status Public on Oct 10, 2015
Title CD4Tcell_RNA_exp2
Sample type SRA
 
Source name Primary CD4_T cells isolated from 6-8 week old mice (C57BL/6)
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: CD4 T cell
Growth protocol Splenic CD4_T cells were purified using magnetic beads and an AutoMACS sorter (Miltenyi Biotec) that yielded purity of >98%.
Extracted molecule total RNA
Extraction protocol Total cellular RNA was extracted with TRIzol reagent (Invitrogen).
TruSeq RNA Sample Prep Kit v2 (Illumina) was used according to the manufacturer's protocol.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description Splenic_CD4_T_cells
Data processing Read sequences (50 bp) were aligned to the mm10 mouse reference genome (University of California, Santa Cruz (UCSC) December 2011) using Bowtie (version 0.12.8) and TopHat (version 1.3.2).
Fragments per kilobase of exon per million mapped reads (FPKM) for each gene were calculated using Cufflinks (version 2.0.2).
Genome_build: mm10
Supplementary_files_format_and_content: Text files include FPKM values for each Sample
 
Submission date Oct 07, 2015
Last update date May 15, 2019
Contact name Atsushi Onodera
Organization name Chiba University
Department Immunology
Street address Chuo-ku Inohana 1-8-1
City Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platform ID GPL17021
Series (2)
GSE73813 Spatial interplay between Polycomb and Trithorax complexes controls transcriptional activity in T lymphocytes [RNA-Seq]
GSE73825 Spatial interplay between Polycomb and Trithorax complexes controls transcriptional activity in T lymphocytes
Relations
BioSample SAMN04151784
SRA SRX1310207

Supplementary file Size Download File type/resource
GSM1903918_CD4T_exp2.txt.gz 253.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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