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Sample GSM1898771 Query DataSets for GSM1898771
Status Public on Oct 01, 2015
Title CSC 2
Sample type RNA
Source name CSCs
Organism Rattus norvegicus
Characteristics background strain: Sprague Dawley
tissue: heart
cell type: cardiac stem cells (CSCs)
age: 4 weeks
Treatment protocol For miRNA array analysis, CSCs were grown in culture medium supplemented with MSC-exosomes for 3 consecutive days. Then cells were collected for RNA extraction and subsequent experiments.
Growth protocol Cardiac stem cells were isolated and cultured as previously reported. MSCs were isolated and cultured until Passage 4. Then exosomes from MSCs were extracted using Exo-Quick-TCTM (System Bioscience) according to the user manual.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Per RNA sample, 1 ug were used as input into the procedure as recommended by protocol (Genisphere FlashTagTM Biotin RNA Labeling for Affymetrix GeneChip miRNA arrays). 1ug of total RNA was used as input into the labeling reaction.
Hybridization protocol Labeled miRNA was hybridized to the array for 16 hours at 48 “C and 60 rpm as described in the protocol.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G
Description miRNA expression profiling from CSCs
Data processing Expression data were generated by Affymetrix Expression Console software and normalized by the MAS5 method.
Submission date Sep 30, 2015
Last update date Oct 01, 2015
Contact name Junjie Yang
Phone 0086-532-67780948
Organization name Institute for cardiovascular science of Soochow University
Street address 708 Renmin Road
City Suzhou
ZIP/Postal code 215006
Country China
Platform ID GPL19117
Series (1)
GSE73598 Expression data from cardiac stem cells (CSCs) with or without MSC-exosomes treatment

Data table header descriptions
VALUE log2 GC-RMA signal

Data table
MIMAT0001619_st 5.418149
MIMAT0000547_st 6.243561
MIMAT0004637_st 0.9089534
MIMAT0000550_st 0.1131742
MIMAT0017026_st -0.128305
MIMAT0000552_st 4.267799
MIMAT0000553_st 9.911139
MIMAT0000554_st 8.42272
MIMAT0000557_st 0.6536121
MIMAT0004639_st 0.4398492
MIMAT0017028_st 5.470766
MIMAT0000560_st 5.610392
MIMAT0000561_st 3.59599
MIMAT0000562_st 13.21456
MIMAT0000563_st 3.832046
MIMAT0017029_st 8.347347
MIMAT0000564_st 7.125108
MIMAT0017031_st 0.4654036
MIMAT0000566_st 2.905713
MIMAT0004641_st 1.575672

Total number of rows: 1277

Table truncated, full table size 30 Kbytes.

Supplementary file Size Download File type/resource
GSM1898771_CSC_2.CEL.gz 763.0 Kb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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