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Sample GSM1898768 Query DataSets for GSM1898768
Status Public on Oct 01, 2015
Title CSC+Exo 2
Sample type RNA
Source name CSCs treated with MSC-exosomes from bone marrow
Organism Rattus norvegicus
Characteristics background strain: Sprague Dawley
tissue: heart
cell type: cardiac stem cells (CSCs)
age: 4 weeks
Treatment protocol For miRNA array analysis, CSCs were grown in culture medium supplemented with MSC-exosomes for 3 consecutive days. Then cells were collected for RNA extraction and subsequent experiments.
Growth protocol Cardiac stem cells were isolated and cultured as previously reported. MSCs were isolated and cultured until Passage 4. Then exosomes from MSCs were extracted using Exo-Quick-TCTM (System Bioscience) according to the user manual.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Per RNA sample, 1 ug were used as input into the procedure as recommended by protocol (Genisphere FlashTagTM Biotin RNA Labeling for Affymetrix GeneChip miRNA arrays). 1ug of total RNA was used as input into the labeling reaction.
Hybridization protocol Labeled miRNA was hybridized to the array for 16 hours at 48 “C and 60 rpm as described in the protocol.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G
Description miRNA expression profiling from CSCs treated with MSC-exosomes
Data processing Expression data were generated by Affymetrix Expression Console software and normalized by the MAS5 method.
Submission date Sep 30, 2015
Last update date Oct 01, 2015
Contact name Junjie Yang
Phone 0086-532-67780948
Organization name Institute for cardiovascular science of Soochow University
Street address 708 Renmin Road
City Suzhou
ZIP/Postal code 215006
Country China
Platform ID GPL19117
Series (1)
GSE73598 Expression data from cardiac stem cells (CSCs) with or without MSC-exosomes treatment

Data table header descriptions
VALUE log2 GC-RMA signal

Data table
MIMAT0001619_st 6.547225
MIMAT0000547_st 6.965734
MIMAT0004637_st 0.3942681
MIMAT0000550_st 0.4434828
MIMAT0017026_st 0.05781393
MIMAT0000552_st 4.044322
MIMAT0000553_st 9.989604
MIMAT0000554_st 8.842177
MIMAT0000557_st 0.7662124
MIMAT0004639_st 0.6181571
MIMAT0017028_st 2.405101
MIMAT0000560_st 6.395595
MIMAT0000561_st 3.16243
MIMAT0000562_st 13.24853
MIMAT0000563_st 3.086884
MIMAT0017029_st 6.755597
MIMAT0000564_st 7.629265
MIMAT0017031_st 0.5278076
MIMAT0000566_st 1.707541
MIMAT0004641_st 2.235635

Total number of rows: 1277

Table truncated, full table size 30 Kbytes.

Supplementary file Size Download File type/resource
GSM1898768_CSC+Exo_2.CEL.gz 742.7 Kb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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