|
| Status |
Public on Sep 01, 2016 |
| Title |
Lhx2_0ng/ml TGFb_2h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Lhx2, untreated, 2h
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Lhx2
treatment: none
|
| Treatment protocol |
Prior to global gene expression analysis, Lhx2 cells were serum-starved (FCS was replaced by 0.5% BSA; StemCell Technologies) over night before treatment with 10 ng/ml TGF-β1 for 2 h.
|
| Growth protocol |
Lhx2 cells were cultured in Iscove's modified Dulbecco’s medium containing L-glutamine and 25 mM HEPES (IMDM; Thermo scientific), supplemented with P/S, 10-4 2-Mercaptoethanol (Sigma-Aldrich), 5% FCS, 100 ng/ml mSCF and 10 ng/ml human interleukin 6 (hIL-6; (PeproTech).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was isolated using Trizol reagent (Invitrogen) according to manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
Agilent’s recommended procedures (Version 5.7, March 2008) were followed for the preparation and labeling of cDNA for microarray-based one-color gene expression analysis.
|
| |
|
| Hybridization protocol |
Agilent’s recommended procedures (Version 5.7, March 2008) were followed for the hybridization and washing of Agilent 60-mer oligonucleotide microarrays.
|
| Scan protocol |
Arrays were scanned using the Agilent microarray scanner. Agilent's recommended procedures (Version 5.7, March 2008) were followed for the scanning and feature extraction of Agilent 60-mer oligonucleotide microarrays for microarray-based one-color gene expression analysis.
|
| Description |
Untreated_2
Gene expression 2 hr after no treatment.
|
| Data processing |
Arrays were normalized using cyclic-loess, and differentially expressed genes were identified using LIMMA (16646809) FDR< 0.2. Expression profiles were clustered using Genesis (11836235).
|
| |
|
| Submission date |
Sep 29, 2015 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Shamit Soneji |
| Organization name |
BMC
|
| Street address |
Solvegatan 19
|
| City |
Lund |
| ZIP/Postal code |
22184 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL7202 |
| Series (2) |
| GSE73567 |
A network including TGFβ/Smad4, Gata2 and p57 regulates proliferation of mouse hematopoietic stem/progenitor cells [expression] |
| GSE73642 |
A network including TGFβ/Smad4, Gata2 and p57 regulates proliferation of mouse hematopoietic stem/progenitor cells |
|
