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Sample GSM1897940 Query DataSets for GSM1897940
Status Public on Feb 17, 2017
Title HFL-1 siNTC, rep1
Sample type SRA
Source name siNTC
Organism Homo sapiens
Characteristics cell line: HFL-1
transfection: siNTC
Treatment protocol Cells were transfected with siRNA, using Lipofectamine RNAiMAX (Invitrogen), according to the manufacturer’s instructions.Total RNA was extracted from HFL-1 cells 48h after transfection.
Growth protocol Standard HFL-1 growth condition.
Extracted molecule total RNA
Extraction protocol RNeasy mini kit (Qiagen) was used for total RNA extraction.
RNA libraries were prepared for sequencing using standard Ion Torrent protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Ion Torrent Proton
Data processing BAM Alignment to Hg19 (TMAP version 3.4.1, the Torrent Mapping Alignment Program for Ion Torrent Data)
Read count analysis (HTseq version 0.6.1, Ensemble annotation​)
Genome_build: Hg19
Supplementary_files_format_and_content: tab-delimited text file include raw read count values for each Sample
Submission date Sep 29, 2015
Last update date May 15, 2019
Contact name Satoshi Noguchi
Organization name Graduate School of Medicine, The University of Tokyo
Department Department of Respiratory Medicine
Street address 7-3-1 Hongo, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
Platform ID GPL17303
Series (1)
GSE73555 Genes regulated by TAZ in a lung fibroblast cell line
BioSample SAMN04121995
SRA SRX1295477

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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