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Sample GSM1892518 Query DataSets for GSM1892518
Status Public on Dec 31, 2018
Title BAL_HV11
Sample type RNA
Source name Healthy Volunteer_BAL cells
Organism Homo sapiens
Characteristics diagnosis: Healthy Volunteer
cohort: Freiburg
age (yrs): 53
gender (male=1): 0
cell type: bronchoalveolar lavage (BAL) cells
Extracted molecule total RNA
Extraction protocol Isolation protocol: BAL cells were obtained by bronchoscopy, immediately isolated and 1 ml of TRIzol was added to the cell pellet, which was then immediately snap frozen in -80 celsius.
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA
Hybridization protocol Following fragmentation, cRNA were hybridized on Affymetrix Whole-Transcript Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using a CGS30007G scanner (serialnumber 54713600)
Description HUGENE-1_0-ST-V1 HV_11
Data processing Raw CEL files were RMA normalized using the R/Bioconductor package oligo (V.1.32.0) with default settings. Gene expression data was normalized to the geometric mean of all samples using quantile normalization and expression levels were summarized.
Submission date Sep 24, 2015
Last update date Dec 31, 2018
Contact name Antje Prasse
Organization name Medical School Hannover
Department Pneumology
Lab Prasse OE6870
Street address Carl-Neubergstrasse 1
City Hannover
ZIP/Postal code 30625
Country Germany
Platform ID GPL10739
Series (2)
GSE70867 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells
GSE73394 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells (III)

Data table header descriptions
VALUE RMA singal intensity

Data table
7892501 4.864130228
7892502 6.082308493
7892503 6.628032486
7892504 10.07138312
7892505 3.840941866
7892506 5.805833384
7892507 6.203938878
7892508 8.405026406
7892509 11.50515854
7892510 5.666242493
7892511 6.582349532
7892512 7.505162361
7892513 5.62732202
7892514 12.24368021
7892515 10.38942639
7892516 6.043703354
7892517 7.643900143
7892518 4.325738678
7892519 5.413051166
7892520 10.21805848

Total number of rows: 257430

Table truncated, full table size 4996 Kbytes.

Supplementary file Size Download File type/resource
GSM1892518_HUGENE-1_0-ST-V1_HV_11.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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