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Sample GSM1892517 Query DataSets for GSM1892517
Status Public on Dec 31, 2018
Title BAL_HV10
Sample type RNA
 
Source name Healthy Volunteer_BAL cells
Organism Homo sapiens
Characteristics diagnosis: Healthy Volunteer
cohort: Freiburg
age (yrs): 24
gender (male=1): 1
cell type: bronchoalveolar lavage (BAL) cells
Extracted molecule total RNA
Extraction protocol Isolation protocol: BAL cells were obtained by bronchoscopy, immediately isolated and 1 ml of TRIzol was added to the cell pellet, which was then immediately snap frozen in -80 celsius.
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA
 
Hybridization protocol Following fragmentation, cRNA were hybridized on Affymetrix Whole-Transcript Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using a CGS30007G scanner (serialnumber 54713600)
Description HUGENE-1_0-ST-V1 HV_10
Data processing Raw CEL files were RMA normalized using the R/Bioconductor package oligo (V.1.32.0) with default settings. Gene expression data was normalized to the geometric mean of all samples using quantile normalization and expression levels were summarized.
 
Submission date Sep 24, 2015
Last update date Dec 31, 2018
Contact name Antje Prasse
Organization name Medical School Hannover
Department Pneumology
Lab Prasse OE6870
Street address Carl-Neubergstrasse 1
City Hannover
ZIP/Postal code 30625
Country Germany
 
Platform ID GPL10739
Series (2)
GSE70867 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells
GSE73394 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells (III)

Data table header descriptions
ID_REF
VALUE RMA singal intensity

Data table
ID_REF VALUE
7892501 5.600668115
7892502 6.021546708
7892503 4.345430303
7892504 10.24701201
7892505 3.885368289
7892506 5.484092303
7892507 4.944249457
7892508 7.032757293
7892509 11.40139106
7892510 5.67943261
7892511 5.961771095
7892512 7.760179942
7892513 4.905014359
7892514 11.91544889
7892515 10.28324145
7892516 5.740330398
7892517 7.687444658
7892518 4.200237101
7892519 5.802685127
7892520 9.961601103

Total number of rows: 257430

Table truncated, full table size 5001 Kbytes.




Supplementary file Size Download File type/resource
GSM1892517_HUGENE-1_0-ST-V1_HV_10.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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