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Sample GSM1892501 Query DataSets for GSM1892501
Status Public on Dec 31, 2018
Title BAL_SARC_20
Sample type RNA
Source name SARC_BAL cells
Organism Homo sapiens
Characteristics diagnosis: SARC
cohort: Freiburg
age (yrs): 55
gender (male=1): 0
cell type: bronchoalveolar lavage (BAL) cells
Extracted molecule total RNA
Extraction protocol Isolation protocol: BAL cells were obtained by bronchoscopy, immediately isolated and 1 ml of TRIzol was added to the cell pellet, which was then immediately snap frozen in -80 celsius.
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA
Hybridization protocol Following fragmentation, cRNA were hybridized on Affymetrix Whole-Transcript Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using a CGS30007G scanner (serialnumber 54713600)
Description HUGENE-1_0-ST-V1 SARC_20
Data processing Raw CEL files were RMA normalized using the R/Bioconductor package oligo (V.1.32.0) with default settings. Gene expression data was normalized to the geometric mean of all samples using quantile normalization and expression levels were summarized.
Submission date Sep 24, 2015
Last update date Dec 31, 2018
Contact name Antje Prasse
Organization name Medical School Hannover
Department Pneumology
Lab Prasse OE6870
Street address Carl-Neubergstrasse 1
City Hannover
ZIP/Postal code 30625
Country Germany
Platform ID GPL10739
Series (2)
GSE70867 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells
GSE73394 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells (III)

Data table header descriptions
VALUE RMA singal intensity

Data table
7892501 4.055966005
7892502 6.261864323
7892503 4.301611035
7892504 9.972791156
7892505 5.512980113
7892506 6.667090535
7892507 6.074243553
7892508 7.209565759
7892509 11.45934003
7892510 4.783215812
7892511 5.146684911
7892512 7.091648997
7892513 5.305390282
7892514 11.22331065
7892515 10.16967595
7892516 4.514546702
7892517 7.113782137
7892518 3.678042435
7892519 5.158916395
7892520 9.95305641

Total number of rows: 257430

Table truncated, full table size 5001 Kbytes.

Supplementary file Size Download File type/resource
GSM1892501_HUGENE-1_0-ST-V1_SARC_20.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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