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Sample GSM1892494 Query DataSets for GSM1892494
Status Public on Dec 31, 2018
Title BAL_SARC_13
Sample type RNA
Source name SARC_BAL cells
Organism Homo sapiens
Characteristics diagnosis: SARC
cohort: Freiburg
age (yrs): 40
gender (male=1): 1
cell type: bronchoalveolar lavage (BAL) cells
Extracted molecule total RNA
Extraction protocol Isolation protocol: BAL cells were obtained by bronchoscopy, immediately isolated and 1 ml of TRIzol was added to the cell pellet, which was then immediately snap frozen in -80 celsius.
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA
Hybridization protocol Following fragmentation, cRNA were hybridized on Affymetrix Whole-Transcript Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using a CGS30007G scanner (serialnumber 54713600)
Description HUGENE-1_0-ST-V1 SARC_13
Data processing Raw CEL files were RMA normalized using the R/Bioconductor package oligo (V.1.32.0) with default settings. Gene expression data was normalized to the geometric mean of all samples using quantile normalization and expression levels were summarized.
Submission date Sep 24, 2015
Last update date Dec 31, 2018
Contact name Antje Prasse
Organization name Medical School Hannover
Department Pneumology
Lab Prasse OE6870
Street address Carl-Neubergstrasse 1
City Hannover
ZIP/Postal code 30625
Country Germany
Platform ID GPL10739
Series (2)
GSE70867 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells
GSE73394 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells (III)

Data table header descriptions
VALUE RMA singal intensity

Data table
7892501 3.742998036
7892502 5.476754564
7892503 4.784135297
7892504 9.888765349
7892505 5.669989716
7892506 6.283160779
7892507 5.612183126
7892508 6.524538871
7892509 11.42630861
7892510 5.988808064
7892511 5.044471398
7892512 7.124842126
7892513 5.516768251
7892514 11.65603521
7892515 9.867927737
7892516 4.733586466
7892517 7.336402269
7892518 3.98328996
7892519 5.390865707
7892520 9.51207948

Total number of rows: 257430

Table truncated, full table size 5001 Kbytes.

Supplementary file Size Download File type/resource
GSM1892494_HUGENE-1_0-ST-V1_SARC_13.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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