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Sample GSM1892491 Query DataSets for GSM1892491
Status Public on Dec 31, 2018
Title BAL_SARC_10
Sample type RNA
 
Source name SARC_BAL cells
Organism Homo sapiens
Characteristics diagnosis: SARC
cohort: Freiburg
age (yrs): 40
gender (male=1): 1
cell type: bronchoalveolar lavage (BAL) cells
Extracted molecule total RNA
Extraction protocol Isolation protocol: BAL cells were obtained by bronchoscopy, immediately isolated and 1 ml of TRIzol was added to the cell pellet, which was then immediately snap frozen in -80 celsius.
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA
 
Hybridization protocol Following fragmentation, cRNA were hybridized on Affymetrix Whole-Transcript Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using a CGS30007G scanner (serialnumber 54713600)
Description HUGENE-1_0-ST-V1 SARC_10
Data processing Raw CEL files were RMA normalized using the R/Bioconductor package oligo (V.1.32.0) with default settings. Gene expression data was normalized to the geometric mean of all samples using quantile normalization and expression levels were summarized.
 
Submission date Sep 24, 2015
Last update date Dec 31, 2018
Contact name Antje Prasse
Organization name Medical School Hannover
Department Pneumology
Lab Prasse OE6870
Street address Carl-Neubergstrasse 1
City Hannover
ZIP/Postal code 30625
Country Germany
 
Platform ID GPL10739
Series (2)
GSE70867 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells
GSE73394 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells (III)

Data table header descriptions
ID_REF
VALUE RMA singal intensity

Data table
ID_REF VALUE
7892501 3.84555656
7892502 5.517424194
7892503 4.85776121
7892504 9.864760131
7892505 4.106236027
7892506 6.14715719
7892507 5.804216899
7892508 6.362136521
7892509 11.25965908
7892510 4.72195233
7892511 5.298620309
7892512 7.76748352
7892513 5.149098439
7892514 11.547143
7892515 9.807480126
7892516 4.423764658
7892517 6.494231651
7892518 3.518213715
7892519 6.650098881
7892520 9.167540512

Total number of rows: 257430

Table truncated, full table size 4998 Kbytes.




Supplementary file Size Download File type/resource
GSM1892491_HUGENE-1_0-ST-V1_SARC_10.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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