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Sample GSM1892482 Query DataSets for GSM1892482
Status Public on Dec 31, 2018
Title BAL_SARC_1
Sample type RNA
Source name SARC_BAL cells
Organism Homo sapiens
Characteristics diagnosis: SARC
cohort: Freiburg
age (yrs): 42
gender (male=1): 1
cell type: bronchoalveolar lavage (BAL) cells
Extracted molecule total RNA
Extraction protocol Isolation protocol: BAL cells were obtained by bronchoscopy, immediately isolated and 1 ml of TRIzol was added to the cell pellet, which was then immediately snap frozen in -80 celsius.
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA
Hybridization protocol Following fragmentation, cRNA were hybridized on Affymetrix Whole-Transcript Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using a CGS30007G scanner (serialnumber 54713600)
Description HUGENE-1_0-ST-V1 SARC_1
Data processing Raw CEL files were RMA normalized using the R/Bioconductor package oligo (V.1.32.0) with default settings. Gene expression data was normalized to the geometric mean of all samples using quantile normalization and expression levels were summarized.
Submission date Sep 24, 2015
Last update date Dec 31, 2018
Contact name Antje Prasse
Organization name Medical School Hannover
Department Pneumology
Lab Prasse OE6870
Street address Carl-Neubergstrasse 1
City Hannover
ZIP/Postal code 30625
Country Germany
Platform ID GPL10739
Series (2)
GSE70867 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells
GSE73394 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells (III)

Data table header descriptions
VALUE RMA singal intensity

Data table
7892501 3.635325236
7892502 5.338806471
7892503 4.980553196
7892504 9.621593635
7892505 4.5060703
7892506 5.709897704
7892507 5.867802895
7892508 6.389796382
7892509 11.23497336
7892510 4.651560226
7892511 5.165029391
7892512 7.80450478
7892513 5.383821468
7892514 11.55378288
7892515 9.293988517
7892516 4.990363175
7892517 7.356506087
7892518 3.818084872
7892519 5.217134445
7892520 9.725498679

Total number of rows: 257430

Table truncated, full table size 4994 Kbytes.

Supplementary file Size Download File type/resource
GSM1892482_HUGENE-1_0-ST-V1_SARC_1.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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