|
Status |
Public on Sep 19, 2015 |
Title |
FD Fetus Brain 1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Fetal brain, maternal control diet
|
Organism |
Mus musculus |
Characteristics |
strain/background: C57BL/6J tissue: fetal brain maternal diet: ad libitum
|
Treatment protocol |
Control (normal diet), FD (Food Deprived for overnight at GD17) treatment diet, FR (Food Restricted from GD10 to 17) treatment diet.
|
Growth protocol |
C57BL/6J mice were purchased from Japan SLC Co. Ltd. (Hamamatsu, Japan). The animals were housed at the Animal Institution in Showa University. They were maintained in cages in a ventilated animal room with controlled temperature and relative humidity with a 12 h light: 12 h dark schedule (lights turned on at 8:00 AM) and had access to chow (CE-2, CLEA Japan) and tap water ad libitum. As per the supplier's (CLEA) information, CE-2 includes 51.4% nitrogen free extracts, 24.9% crude protein, 8.6% moisture, 6.7% crude ash, 4.6% crude fat, and 3.7% crude fiber. The physiological fuel value is 346.7 kcal per 100 g chow. All animal experiments were started after a period of acclimation of at least one week. Pregnant animals were obtained by housing females with males (1-2 females/male). The day the vaginal plug was observed was designated embryonic day 0 (ED0) and gestation day 0 (GD0).
|
Extracted molecule |
total RNA |
Extraction protocol |
Kit: QIAGEN, RNeasy Mini
|
Label |
Cy3
|
Label protocol |
Manual: Low RNA Input Linear Amplification kit standard protocol
|
|
|
Channel 2 |
Source name |
Fetal brain, maternal food deprivation
|
Organism |
Mus musculus |
Characteristics |
strain/background: C57BL/6J tissue: fetal brain maternal diet: food deprivation
|
Treatment protocol |
Control (normal diet), FD (Food Deprived for overnight at GD17) treatment diet, FR (Food Restricted from GD10 to 17) treatment diet.
|
Growth protocol |
C57BL/6J mice were purchased from Japan SLC Co. Ltd. (Hamamatsu, Japan). The animals were housed at the Animal Institution in Showa University. They were maintained in cages in a ventilated animal room with controlled temperature and relative humidity with a 12 h light: 12 h dark schedule (lights turned on at 8:00 AM) and had access to chow (CE-2, CLEA Japan) and tap water ad libitum. As per the supplier's (CLEA) information, CE-2 includes 51.4% nitrogen free extracts, 24.9% crude protein, 8.6% moisture, 6.7% crude ash, 4.6% crude fat, and 3.7% crude fiber. The physiological fuel value is 346.7 kcal per 100 g chow. All animal experiments were started after a period of acclimation of at least one week. Pregnant animals were obtained by housing females with males (1-2 females/male). The day the vaginal plug was observed was designated embryonic day 0 (ED0) and gestation day 0 (GD0).
|
Extracted molecule |
total RNA |
Extraction protocol |
Kit: QIAGEN, RNeasy Mini
|
Label |
Cy5
|
Label protocol |
Manual: Low RNA Input Linear Amplification kit standard protocol
|
|
|
|
Hybridization protocol |
Hybridization kit: in situ hybridization plus
|
Scan protocol |
Scanner: Agilent DNA microarray Scanner G2505C
|
Description |
Dye-swap (Cy3/Cy5) of Control vs. FD Fetus Brain Control Fetus Brain (Cy3) x FD Fetus Brain (Cy5)
|
Data processing |
Software: Feature Extraction ver 9.5.3.1 Normalization: LOWESS (locally weighted linear regression)
|
|
|
Submission date |
Sep 18, 2015 |
Last update date |
Sep 19, 2015 |
Contact name |
RANDEEP RAKWAL |
E-mail(s) |
plantproteomics@gmail.com
|
Phone |
+81-(0)90-1853-7875
|
Organization name |
University of Tsukuba
|
Department |
Institute of Health and Sport Sciences
|
Lab |
Global Sport Innovation (GSI) 403
|
Street address |
1-1-1 Tennodai
|
City |
Tsukuba |
State/province |
Ibaraki |
ZIP/Postal code |
305-8574 |
Country |
Japan |
|
|
Platform ID |
GPL4134 |
Series (1) |
GSE73202 |
Seeking genes responsible for developmental origins of health and disease (DOHaD) from the fetal mouse brain following maternal food deprivation (FD) and food restriction (FR) using OMICS approach |
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