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Sample GSM1888492 Query DataSets for GSM1888492
Status Public on Sep 19, 2015
Title FD Fetus Brain 1
Sample type RNA
 
Channel 1
Source name Fetal brain, maternal control diet
Organism Mus musculus
Characteristics strain/background: C57BL/6J
tissue: fetal brain
maternal diet: ad libitum
Treatment protocol Control (normal diet), FD (Food Deprived for overnight at GD17) treatment diet, FR (Food Restricted from GD10 to 17) treatment diet.
Growth protocol C57BL/6J mice were purchased from Japan SLC Co. Ltd. (Hamamatsu, Japan). The animals were housed at the Animal Institution in Showa University. They were maintained in cages in a ventilated animal room with controlled temperature and relative humidity with a 12 h light: 12 h dark schedule (lights turned on at 8:00 AM) and had access to chow (CE-2, CLEA Japan) and tap water ad libitum. As per the supplier's (CLEA) information, CE-2 includes 51.4% nitrogen free extracts, 24.9% crude protein, 8.6% moisture, 6.7% crude ash, 4.6% crude fat, and 3.7% crude fiber. The physiological fuel value is 346.7 kcal per 100 g chow. All animal experiments were started after a period of acclimation of at least one week. Pregnant animals were obtained by housing females with males (1-2 females/male). The day the vaginal plug was observed was designated embryonic day 0 (ED0) and gestation day 0 (GD0).
Extracted molecule total RNA
Extraction protocol Kit: QIAGEN, RNeasy Mini
Label Cy3
Label protocol Manual: Low RNA Input Linear Amplification kit standard protocol
 
Channel 2
Source name Fetal brain, maternal food deprivation
Organism Mus musculus
Characteristics strain/background: C57BL/6J
tissue: fetal brain
maternal diet: food deprivation
Treatment protocol Control (normal diet), FD (Food Deprived for overnight at GD17) treatment diet, FR (Food Restricted from GD10 to 17) treatment diet.
Growth protocol C57BL/6J mice were purchased from Japan SLC Co. Ltd. (Hamamatsu, Japan). The animals were housed at the Animal Institution in Showa University. They were maintained in cages in a ventilated animal room with controlled temperature and relative humidity with a 12 h light: 12 h dark schedule (lights turned on at 8:00 AM) and had access to chow (CE-2, CLEA Japan) and tap water ad libitum. As per the supplier's (CLEA) information, CE-2 includes 51.4% nitrogen free extracts, 24.9% crude protein, 8.6% moisture, 6.7% crude ash, 4.6% crude fat, and 3.7% crude fiber. The physiological fuel value is 346.7 kcal per 100 g chow. All animal experiments were started after a period of acclimation of at least one week. Pregnant animals were obtained by housing females with males (1-2 females/male). The day the vaginal plug was observed was designated embryonic day 0 (ED0) and gestation day 0 (GD0).
Extracted molecule total RNA
Extraction protocol Kit: QIAGEN, RNeasy Mini
Label Cy5
Label protocol Manual: Low RNA Input Linear Amplification kit standard protocol
 
 
Hybridization protocol Hybridization kit: in situ hybridization plus
Scan protocol Scanner: Agilent DNA microarray Scanner G2505C
Description Dye-swap (Cy3/Cy5) of Control vs. FD Fetus Brain
Control Fetus Brain (Cy3) x FD Fetus Brain (Cy5)
Data processing Software: Feature Extraction ver 9.5.3.1
Normalization: LOWESS (locally weighted linear regression)
 
Submission date Sep 18, 2015
Last update date Sep 19, 2015
Contact name RANDEEP RAKWAL
E-mail(s) plantproteomics@gmail.com
Phone +81-(0)90-1853-7875
Organization name University of Tsukuba
Department Institute of Health and Sport Sciences
Lab Global Sport Innovation (GSI) 403
Street address 1-1-1 Tennodai
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8574
Country Japan
 
Platform ID GPL4134
Series (1)
GSE73202 Seeking genes responsible for developmental origins of health and disease (DOHaD) from the fetal mouse brain following maternal food deprivation (FD) and food restriction (FR) using OMICS approach

Data table header descriptions
ID_REF
VALUE Log10 based (normalized ch2 intensity / normalized ch1 intensity)

Data table
ID_REF VALUE
1 -0.307816206
2 -1.146419136
3 -1.140296826
4 -1.134527313
5 -1.129424828
6 -1.124263868
7 -1.119983245
8 -1.116153178
9 -1.112684895
10 -1.109674756
11 -1.107239481
12 -1.104841314
13 0.070431541
14 -0.357442148
15 0.339536060
16 0.014299003
18 -1.096126816
19 -0.143602447
20 0.233143068
21 0.103758147

Total number of rows: 45018

Table truncated, full table size 804 Kbytes.




Supplementary file Size Download File type/resource
GSM1888492_US45102848_251486831692_S01_GE2-v5_95_Feb07_1_1.txt.gz 15.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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