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Sample GSM1888403 Query DataSets for GSM1888403
Status Public on Jun 12, 2017
Title Rif5C vs WtC
Sample type RNA
 
Channel 1
Source name ATCC 17978 derivative spontaneous mutant Rif5
Organism Acinetobacter baumannii
Characteristics strain: ATCC 17978
genotype: spontaneous mutant Rif5
Treatment protocol All strains were grown until the mid-exponential growth phase (OD600=0.6) was reached
Growth protocol A. baumannii ATCC 17978 wild-type strain was grown in Luria Bertani (LB) medium and incubated at 37ºC with shaking at 180 rpm.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using an RNeasy mini kit (Qiagen). DNA contaminants were removed from the RNA by digestion with DNase Turbo (Ambion).
Label Cy5
Label protocol Two-Color Microarray-Based Prokaryote Analysis Fair Play III Labeling v. 1.3 (Agilent)
 
Channel 2
Source name ATCC 17978 wild-type
Organism Acinetobacter baumannii
Characteristics strain: ATCC 17978
genotype: wild-type
Treatment protocol All strains were grown until the mid-exponential growth phase (OD600=0.6) was reached
Growth protocol A. baumannii ATCC 17978 wild-type strain was grown in Luria Bertani (LB) medium and incubated at 37ºC with shaking at 180 rpm.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using an RNeasy mini kit (Qiagen). DNA contaminants were removed from the RNA by digestion with DNase Turbo (Ambion).
Label Cy3
Label protocol Two-Color Microarray-Based Prokaryote Analysis Fair Play III Labeling v. 1.3 (Agilent)
 
 
Hybridization protocol Microarrays were hybridized and washed according to manufacturer's protocol.
Scan protocol Two-Color Microarray-Based Prokaryote Analysis 1.4
Data processing Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1)
 
Submission date Sep 18, 2015
Last update date Jun 13, 2017
Contact name Jesús Aranda
E-mail(s) jesus.aranda@uab.cat
Organization name Universitat Autònoma de Barcelona
Department Departament de Genètica i Microbiologia
Street address Facultat de Biociències, Plaza Cívica, s/n
City Barcelona
ZIP/Postal code 08193
Country Spain
 
Platform ID GPL16729
Series (1)
GSE73193 Characterization of two Acinetobacter baumannii rifampin-spontaneous mutants lacking twitching motility

Data table header descriptions
ID_REF
VALUE Background corrected and normalized with loess and Aquantile log2 ratio (Cy5/Cy3)

Data table
ID_REF VALUE
1 0.781288619
2 -0.033719544
3 0.156257843
4 0.359694747
5 0.269668365
6 0.471132888
7 0.102444458
8 0.574664287
9 -0.025714738
10 -0.026497795
11 -0.429970949
12 0.200842628
13 0.112168849
14 -0.457664702
15 0.312428431
16 -0.048863454
17 0.370885467
18 0.113421951
19 0.159433341
20 0.075413695

Total number of rows: 15744

Table truncated, full table size 271 Kbytes.




Supplementary file Size Download File type/resource
GSM1888403_G.txt.gz 1.5 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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