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Sample GSM1888137 Query DataSets for GSM1888137
Status Public on Sep 19, 2015
Title Patient 10, CD8+ cells, before second dose of fingolimod [exon-level]
Sample type RNA
 
Source name Peripheral blood CD8+ cells
Organism Homo sapiens
Characteristics patient identifier: FCCI-7765
gender: male
age in years at baseline: 37
disease duration in years at baseline: 9
previous treatment: glatiramer acetate
treatment gap in months: 1
edss at baseline: 5.5
edss after 1 year: 4.0
edss after 2 years: 3.5
edss after 3 years: 3.5
edss after 4 years: 3.5
relapses during the year prior to fingolimod: 1
relapses during 1-year follow-up: 0
relapses during 2-year follow-up: 0
relapses during 3-year follow-up: 0
relapses during 4-year follow-up: 0
completed years of fingolimod therapy: >=4
Treatment protocol Patients were treated with fingolimod (Gilenya, Novartis) according to the approved label (0.5 mg orally once-daily) and the guidelines and recommendations of the German Society of Neurology.
Growth protocol Patient blood samples were taken immediately before the first dose of fingolimod as well as one day and three months post therapy initiation.
Extracted molecule total RNA
Extraction protocol Peripheral blood CD8+ cells were separated by magnetic-activated cell sorting using Whole Blood CD8 MicroBeads (Miltenyi Biotec) and total RNA was isolated using the mirVana isolation kit (Thermo Fisher Scientific) according to the manufacturers' protocols.
Label Biotin
Label protocol According to the Affymetrix Whole Transcript (WT) manual, cRNA was prepared from 200 ng total RNA. The cRNA was then used to generate single-stranded DNA, which was fragmented and biotinylated.
 
Hybridization protocol Labeled single-stranded DNA in the sense orientation was hybridized for 16 hours at 45 °C on Affymetrix HTA 2.0 microarrays. The microarrays were washed and stained with a streptavidin-phycoerythrin conjugate in an Affymetrix Fluidics Station 450. Signal amplification with antibodies was applied following the instructions provided by Affymetrix.
Scan protocol The microarrays were scanned with a GeneChip Scanner 3000 7G (Affymetrix).
Description Gene expression data from a multiple sclerosis patient treated with fingolimod
Data processing Data preprocessing of the raw microarray scans was performed using the Affymetrix GeneChip Command Console (AGCC) software version 4.0. The data were then processed using Expression Console version 1.3.1. The robust multi-array average (RMA) algorithm was applied with the default configuration, which includes a log2 data transformation and quantile normalization. In this step, the measured signal intensities of >6 million probes were summarized into gene level probe sets (n=70523) and exon level probe sets (n=914585). The Transcriptome Analysis Console (TAC) software version 1.0 was utilized to analyze the RNA expression dynamics.
 
Submission date Sep 18, 2015
Last update date Mar 23, 2018
Contact name Michael Hecker
E-mail(s) michael.hecker@rocketmail.com
Organization name University of Rostock
Department Department of Neurology
Lab Division of Neuroimmunology
Street address Gehlsheimer Str. 20
City Rostock
ZIP/Postal code 18147
Country Germany
 
Platform ID GPL17585
Series (2)
GSE73172 Transcriptome data of multiple sclerosis patients receiving fingolimod therapy [HTA-2_0, CD8+ cells, exon level]
GSE73174 Transcriptome data of multiple sclerosis patients receiving fingolimod therapy
Relations
Alternative to GSM1885041 (gene-level analysis)

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
JUC01000001.hg.1 5.480233
JUC01000002.hg.1 4.115462
JUC01000003.hg.1 5.755018
JUC01000004.hg.1 6.183787
JUC01000005.hg.1 6.934806
JUC01000006.hg.1 6.807576
JUC01000007.hg.1 2.708848
JUC01000008.hg.1 4.650693
JUC01000009.hg.1 2.361791
JUC01000010.hg.1 2.277456
JUC01000011.hg.1 11.01295
JUC01000012.hg.1 7.790661
JUC01000013.hg.1 10.25037
JUC01000014.hg.1 8.34955
JUC01000015.hg.1 5.574989
JUC01000016.hg.1 7.353043
JUC01000017.hg.1 5.540529
JUC01000018.hg.1 7.648423
JUC01000019.hg.1 7.251081
JUC01000020.hg.1 7.251081

Total number of rows: 914585

Table truncated, full table size 23428 Kbytes.




Supplementary file Size Download File type/resource
GSM1888137_CD8_Pat10_24_hours.CEL.gz 21.4 Mb (ftp)(http) CEL
GSM1888137_CD8_Pat10_24_hours.rma-alt-splice-dabg.chp.gz 9.3 Mb (ftp)(http) CHP
Processed data included within Sample table

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