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Sample GSM1886346 Query DataSets for GSM1886346
Status Public on Jul 12, 2016
Title chameau KD-1
Sample type SRA
Source name embryos
Organism Drosophila melanogaster
Characteristics cell line: S2
genotype/variation: chameau KD
Growth protocol S2 cells were grown in Schneider’s Drosophila media (Gibco) with 10% FBS.
Extracted molecule total RNA
Extraction protocol S2 cells were collected.Total RNA was isolated using ISOGEN II reagent (NIPPON GENE), according to the manufacturer’s protocol.
RNA-seq libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep kit (Illumina)
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina MiSeq
Description Sample 3
Data processing Basecalls performed using Illumina MiSeq
RNA-seq reads were aligned to the Release 5 (dm3) genome assembly using Illumina MiSeq
Partek genomic suite was used to generate RPKMs. Quantification was performed using transcript-level and a significant signal was filtered by including at least one of the signal having more than one RPKM.
Genome_build: Release 5 (dm3)
Supplementary_files_format_and_content: RPKM
Submission date Sep 16, 2015
Last update date May 15, 2019
Organization name Nagasaki unversity
Department Biochemistry
Street address 1-12-4 sakamoto
City Nagasaki
ZIP/Postal code 852-8523
Country Japan
Platform ID GPL16479
Series (1)
GSE73098 Chameau Activates Transcription with Enhancer of Acetyltransferase Chameau (EAChm) in vitro
Reanalyzed by GSM3276733
BioSample SAMN04090375
SRA SRX1250574

Supplementary file Size Download File type/resource
GSM1886346_GSM03_ChmKD_S3_RPKM.txt.gz 154.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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