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Sample GSM1876145 Query DataSets for GSM1876145
Status Public on Sep 15, 2016
Title FKB_3
Sample type RNA
Source name HeLa Cells, 48h FKB-Treated, replicate 3
Organism Homo sapiens
Characteristics cell line: HeLa
treatment: 48 hours of treatment with FKB: Flavokawain B
Treatment protocol FKB-treated cells were treated with 7µg/mL of FKB for 48 hours, FIH-treated cells were treated with 15 µg/mL of FIH for 48 hours, the untreated cells were treated with the vehicle.
Growth protocol HeLa cells were grown in RPMI-1640 supplemented with 10%FBS and 1% pennicillin-streptomycin and kept in a humidified 37 C chamber equipped with 5%CO2
Extracted molecule total RNA
Extraction protocol Cells were extracted using the QIAGEN Rneasy Mini Kit as per manufacturer's protocol
Label Cy3
Label protocol Cy3labeled cRNA was prepared from 25 ng of total RNA using the Low Input Quick Amp Labelinh One-Color Microarray-Based Gene Expression Analysis
Hybridization protocol 600 ng of Cy3-labeled cRNA (specific activity >6 pmol Cy3/µg cRNA) were fragemnted at 60C for 30 minutes in reaction volume of 25 µl with 1x Agilent Fragmentation Buffer and 2x Agilent blocking agent per manufacturer's protocol
Scan protocol slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (SureScan Microarray Scanner with the product number: G4900DA) using one color scan setting for 8X60k with scan area 61x21.6 mm and 3µm resolution. We used one color channel for Cy3
Description Gene expression in HeLa cells after 48 hours of treatment with FKB
Data processing The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol GE1-v1_91 and Grid: 012391_D_20060331) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
Submission date Sep 13, 2015
Last update date Apr 23, 2018
Contact name Nadiah Abu
Phone +60135103138
Organization name Universiti Putra Malaysia
Department Faculty of Biotechnology
Street address Animal Tissue Culture Lab
City Serdang
State/province Selangor
ZIP/Postal code 43400
Country Malaysia
Platform ID GPL17077
Series (1)
GSE72974 Gene expression analysis reveals the concurrent activation of pro-apoptotic and antioxidant-defensive mechanisms in Flavokawain B and FIH-treated cervical cancer HeLa cells
Reanalyzed by GSE113533

Data table header descriptions
VALUE normalized signal intensity

Data table
(+)E1A_r60_1 0.18530655
(+)E1A_r60_3 0.1196146
(+)E1A_r60_a104 0
(+)E1A_r60_a107 0.037778616
(+)E1A_r60_a135 0.08684349
(+)E1A_r60_a20 0.12661457
(+)E1A_r60_a22 0.18379688
(+)E1A_r60_a97 0.12833977
(+)E1A_r60_n11 0.10896969
(+)E1A_r60_n9 0.12890911
3xSLv1 0
A_19_P00315452 2.7543383
A_19_P00315459 0.7525382
A_19_P00315482 -0.7253485
A_19_P00315492 -1.3440628
A_19_P00315493 0.27007413
A_19_P00315502 0.54726887
A_19_P00315506 0
A_19_P00315518 0
A_19_P00315519 -0.015272141

Total number of rows: 50542

Table truncated, full table size 1159 Kbytes.

Supplementary file Size Download File type/resource
GSM1876145_FKB3_HELA.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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