|
Status |
Public on Nov 25, 2015 |
Title |
CMP_CD41_M |
Sample type |
SRA |
|
|
Source name |
CMP CD41
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 age: 6-8 wks tissue: bone marrow cell type: common myeloid progenitor (CMP) cell subtype: CMP CD41 chip antibody: K4me2 (Abcam-ab7766)
|
Treatment protocol |
no treatment
|
Growth protocol |
6 to 8 weeks-old mice housed at the Weizmann Institute animal facility
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Lara-Astiaso D, Weiner A, Lorenzo-Vivas E, Zaretsky I et al. Immunogenetics. Chromatin state dynamics during blood formation. Science 2014 Aug 22;345(6199):943-9. PMID: 25103404 indexing-first chromatin immunoprecipitation
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
bcl2fastq/2.15.0.4 alignment using bowtiebowtie2/2.1.0 using default parameters Genome_build: mm9 Supplementary_files_format_and_content: bigWig
|
|
|
Submission date |
Sep 09, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Ido Amit |
E-mail(s) |
ido.amit@weizmann.ac.il
|
Phone |
972-8-9343338
|
Organization name |
Weizmann Institute of Science
|
Department |
Immunology
|
Street address |
234 Herzl st.
|
City |
Rehovot |
ZIP/Postal code |
760001 |
Country |
Israel |
|
|
Platform ID |
GPL19057 |
Series (2) |
GSE72858 |
Transcriptional heterogeneity and lineage commitment in myeloid progenitors [ChIP-seq] |
GSE72859 |
Transcriptional heterogeneity and lineage commitment in myeloid progenitors |
|
Relations |
BioSample |
SAMN04044122 |
SRA |
SRX1213159 |