GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1868659 Query DataSets for GSM1868659
Status Public on Mar 01, 2016
Title Sarcoma iPSC#2-B5
Sample type RNA
Source name sarcoma-derived iPSCs
Organism Mus musculus
Characteristics cell type: sarcoma-derived iPSCs
Treatment protocol SCOS#2 and #12 Dox ON samples were treated with Dox (2µg/ml) containing medium for 72hours. SCOS#2 and #12 Dox OFF samples were mainteined with Dox-free medium for 72hours. RNA of sarcoma iPSCs was collected after removal of feeder cells (MEF).
Growth protocol DMEM+10%FBS+PS (SCOS#2 and #12) / Knockout DMEM+15%FBS+PS+L-glutamine+βME+LIF (Sarcoma iPSC)
Extracted molecule total RNA
Extraction protocol RNeasy Plus Mini kit
Label Biotin
Label protocol Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99℃ for 5 minutes, then 45℃ for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 80μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 17 hours at 45° at 60rpm• Fluidics washing is FS450_0007
Scan protocol Affymetrix Gene ChIP Scanner 3000 TG
Data processing Data were processed using Genespring software.
Submission date Sep 03, 2015
Last update date Mar 01, 2016
Contact name Yasuhiro Yamada
Organization name University of Tokyo
Department Department of Molecular Pathology
Street address 7-3-1 Hongo, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
Platform ID GPL6246
Series (2)
GSE72704 EWS-FLI1-induced osteosarcoma model unveiled a crucial role of impaired osteogenic differentiation on osteosarcoma development [gene expression]
GSE72898 EWS-FLI1-induced osteosarcoma model unveiled a crucial role of impaired osteogenic differentiation on osteosarcoma development

Data table header descriptions
VALUE Quantile normalized gene level expression values from Genespring GX

Data table
10344614 490.9306
10344616 24.817722
10344618 27.486248
10344620 54.139534
10344622 455.31613
10344624 4292.9526
10344633 2831.1812
10344637 921.7203
10344653 41.93666
10344658 408.66965
10344674 43.085594
10344679 88.66756
10344705 101.47329
10344707 469.4185
10344713 5833.6885
10344715 52.766422
10344717 32.561905
10344719 225.93495
10344721 24.319998
10344723 473.12558

Total number of rows: 28856

Table truncated, full table size 525 Kbytes.

Supplementary file Size Download File type/resource
GSM1868659_Sarcoma_iPSC__2-B5_MoGene-1_0-st-v1_.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap