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Sample GSM1868658 Query DataSets for GSM1868658
Status Public on Mar 01, 2016
Title Sarcoma iPSC#2-A1
Sample type RNA
Source name sarcoma-derived iPSCs
Organism Mus musculus
Characteristics cell type: sarcoma-derived iPSCs
Treatment protocol SCOS#2 and #12 Dox ON samples were treated with Dox (2µg/ml) containing medium for 72hours. SCOS#2 and #12 Dox OFF samples were mainteined with Dox-free medium for 72hours. RNA of sarcoma iPSCs was collected after removal of feeder cells (MEF).
Growth protocol DMEM+10%FBS+PS (SCOS#2 and #12) / Knockout DMEM+15%FBS+PS+L-glutamine+βME+LIF (Sarcoma iPSC)
Extracted molecule total RNA
Extraction protocol RNeasy Plus Mini kit
Label Biotin
Label protocol Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99℃ for 5 minutes, then 45℃ for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 80μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 17 hours at 45° at 60rpm• Fluidics washing is FS450_0007
Scan protocol Affymetrix Gene ChIP Scanner 3000 TG
Data processing Data were processed using Genespring software.
Submission date Sep 03, 2015
Last update date Mar 01, 2016
Contact name Yasuhiro Yamada
Organization name University of Tokyo
Department Department of Molecular Pathology
Street address 7-3-1 Hongo, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
Platform ID GPL6246
Series (2)
GSE72704 EWS-FLI1-induced osteosarcoma model unveiled a crucial role of impaired osteogenic differentiation on osteosarcoma development [gene expression]
GSE72898 EWS-FLI1-induced osteosarcoma model unveiled a crucial role of impaired osteogenic differentiation on osteosarcoma development

Data table header descriptions
VALUE Quantile normalized gene level expression values from Genespring GX

Data table
10344614 1167.4288
10344616 24.649864
10344618 29.220104
10344620 51.387554
10344622 431.62253
10344624 4525.411
10344633 2910.43
10344637 958.33636
10344653 43.816185
10344658 350.00153
10344674 56.692734
10344679 78.02745
10344705 83.18991
10344707 425.87573
10344713 6210.9263
10344715 48.652702
10344717 33.866913
10344719 202.39189
10344721 23.93049
10344723 695.2055

Total number of rows: 28856

Table truncated, full table size 525 Kbytes.

Supplementary file Size Download File type/resource
GSM1868658_Sarcoma_iPSC__2-A1_MoGene-1_0-st-v1_.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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