GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1868656 Query DataSets for GSM1868656
Status Public on Mar 01, 2016
Title SCOS#12 Dox OFF 72h
Sample type RNA
Source name osteosarcoma cell lines
Organism Mus musculus
Characteristics cell line: SCOS#12
cell type: osteosarcoma cell lines
treatment: none
Treatment protocol SCOS#2 and #12 Dox ON samples were treated with Dox (2µg/ml) containing medium for 72hours. SCOS#2 and #12 Dox OFF samples were mainteined with Dox-free medium for 72hours. RNA of sarcoma iPSCs was collected after removal of feeder cells (MEF).
Growth protocol DMEM+10%FBS+PS (SCOS#2 and #12) / Knockout DMEM+15%FBS+PS+L-glutamine+βME+LIF (Sarcoma iPSC)
Extracted molecule total RNA
Extraction protocol RNeasy Plus Mini kit
Label Biotin
Label protocol Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99℃ for 5 minutes, then 45℃ for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 80μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 17 hours at 45° at 60rpm• Fluidics washing is FS450_0007
Scan protocol Affymetrix Gene ChIP Scanner 3000 TG
Data processing Data were processed using Genespring software.
Submission date Sep 03, 2015
Last update date Mar 01, 2016
Contact name Yasuhiro Yamada
Organization name University of Tokyo
Department Department of Molecular Pathology
Street address 7-3-1 Hongo, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
Platform ID GPL6246
Series (2)
GSE72704 EWS-FLI1-induced osteosarcoma model unveiled a crucial role of impaired osteogenic differentiation on osteosarcoma development [gene expression]
GSE72898 EWS-FLI1-induced osteosarcoma model unveiled a crucial role of impaired osteogenic differentiation on osteosarcoma development

Data table header descriptions
VALUE Quantile normalized gene level expression values from Genespring GX

Data table
10344614 141.29271
10344616 24.681778
10344618 25.199356
10344620 50.82862
10344622 259.15344
10344624 2915.062
10344633 2475.899
10344637 831.333
10344653 47.53611
10344658 990.08966
10344674 75.37474
10344679 73.71048
10344705 74.1186
10344707 624.9461
10344713 4115.7
10344715 45.90615
10344717 34.51023
10344719 212.1868
10344721 23.790415
10344723 163.54015

Total number of rows: 28856

Table truncated, full table size 525 Kbytes.

Supplementary file Size Download File type/resource
GSM1868656_SCOS_12_Dox_OFF_72h_MoGene-1_0-st-v1_.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap