|
| Status |
Public on Mar 01, 2016 |
| Title |
SCOS#12 Dox OFF 72h |
| Sample type |
RNA |
| |
|
| Source name |
osteosarcoma cell lines
|
| Organism |
Mus musculus |
| Characteristics |
cell line: SCOS#12
cell type: osteosarcoma cell lines
treatment: none
|
| Treatment protocol |
SCOS#2 and #12 Dox ON samples were treated with Dox (2µg/ml) containing medium for 72hours. SCOS#2 and #12 Dox OFF samples were mainteined with Dox-free medium for 72hours. RNA of sarcoma iPSCs was collected after removal of feeder cells (MEF).
|
| Growth protocol |
DMEM+10%FBS+PS (SCOS#2 and #12) / Knockout DMEM+15%FBS+PS+L-glutamine+βME+LIF (Sarcoma iPSC)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plus Mini kit
|
| Label |
Biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99℃ for 5 minutes, then 45℃ for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 80μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 17 hours at 45° at 60rpm• Fluidics washing is FS450_0007
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 TG
|
| Data processing |
Data were processed using Genespring software.
|
| |
|
| Submission date |
Sep 03, 2015 |
| Last update date |
Mar 01, 2016 |
| Contact name |
Yasuhiro Yamada |
| E-mail(s) |
yyamada@m.u-tokyo.ac.jp
|
| Organization name |
University of Tokyo
|
| Department |
Department of Molecular Pathology
|
| Street address |
7-3-1 Hongo, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE72704 |
EWS-FLI1-induced osteosarcoma model unveiled a crucial role of impaired osteogenic differentiation on osteosarcoma development [gene expression] |
| GSE72898 |
EWS-FLI1-induced osteosarcoma model unveiled a crucial role of impaired osteogenic differentiation on osteosarcoma development |
|
