|
| Status |
Public on Sep 03, 2015 |
| Title |
siRNA-mideiated ARID1A down-regulated |
| Sample type |
RNA |
| |
|
| Source name |
human invasive breast cancer cells MDA-MB-157 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: human invasive breast cancer cells MDA-MB-157 cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNAs were extracted using Rneasy mini kit (Qiagen) accoring to the the manufacturer's recommendations.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 600ng RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
1.5 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 250 ml containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 250 ml of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Human Genome Oligo Microarrays (G4112A) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
|
| Scan protocol |
scanned by Agilent Technologies Micorarrray Scanner and calculated using Agilent Feature 10.7.3.1.
|
| Description |
MOK indicates control siRNA treated cells, while A indicates siRNA-mideiated ARID1A down-regulated cells.
|
| Data processing |
import to GeneSpring GX
|
| |
|
| Submission date |
Sep 02, 2015 |
| Last update date |
Apr 23, 2018 |
| Contact name |
Tamotsu Takeuchi |
| E-mail(s) |
takeutit08@gmail.com
|
| Organization name |
Gifu University School of Medcine
|
| Department |
Pathology and Translational Research
|
| Street address |
Yanagido
|
| City |
Gifu |
| ZIP/Postal code |
501-1194 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16699 |
| Series (1) |
| GSE72669 |
Genes induced by partial down-regulation of ARID1A in breast cancer cell |
|
| Relations |
| Reanalyzed by |
GSE113533 |
