|
| Status |
Public on Aug 09, 2016 |
| Title |
miR-320c_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
primary fibroblast
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary fibroblast
treatment: Cells transfected with miRNA-320c mimic
|
| Treatment protocol |
Primary fibroblast were transfected with scrambled, miR-211 or miR-320c mimic
|
| Growth protocol |
Primary human dermal fibroblasts were isolated from neonatal foreskins (see methods), plated onto collagen dishes and cultured in DMEM supplemented with 10% FBS, 1% penicillin-streptomycin, and 1% L-glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
WT terminal labeling and controls kit (Affymetrix, cat# 901524) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
GeneAtlas Hybridization, Wash and Stain kit for WT array strips (Affymetrix, cat# 901667) was used according to the manufacturer's instructions. Hybridization instrument Accublock Digital Dry Bath (LabNet, D1200) this instrument is adjust for the GeneAtlas microarray.
|
| Scan protocol |
Washing and scanning were performed in GeneAtlas instrument (Affymetrix, 00-0377 (washing fluidic station), 00-0376 (imagine station)).
|
| Data processing |
Raw data (CEL files) was processed using the Chipster software (http://chipster.csc.fi/); normalized expression values were calculated with the RMA algorithm and default parameters
|
| |
|
| Submission date |
Sep 01, 2015 |
| Last update date |
Aug 09, 2016 |
| Contact name |
Carmit Levy |
| E-mail(s) |
carmitlevy@post.tau.ac.il
|
| Organization name |
Tel Aviv University
|
| Department |
Human Genetics and Biochemistry
|
| Lab |
Sackler School of Medicine
|
| Street address |
Ramat Aviv
|
| City |
Tel Aviv |
| ZIP/Postal code |
69978 |
| Country |
Israel |
| |
|
| Platform ID |
GPL20880 |
| Series (2) |
| GSE72619 |
Expression profiling of fibroblasts treated with miR-211 and miR-320c |
| GSE72620 |
Melanoma miRNA trafficking triggers the tumor primary niche formation |
|
