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Sample GSM1862824 Query DataSets for GSM1862824
Status Public on Nov 13, 2016
Title WT_Epidermis_Neonate_replicate2
Sample type RNA
 
Source name WT, Epidermis, Neonate, replicate2
Organism Mus musculus
Characteristics genotype/variation: wild type
tissue: epidermis
strain: mix
age: postnatal day 0
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from epidermis using RNeasy Micro kit (Qiagen).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using the Quick Amp Labeling kit (Agilent) according to the manufacturer's protocol.
 
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Description Gene expression in wild-type epidermis at postnatal day 0, replicate2
Data processing The data were normalized by percentile shift, using GeneSpring GX software (Silicon Genetics).
 
Submission date Aug 27, 2015
Last update date Nov 13, 2016
Contact name Eri H Kobayashi
Organization name Tohoku University Graduate School of Medicine
Department Medical Biochemistry
Street address 2-1 Seiryo-machi, Aobaku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
 
Platform ID GPL7202
Series (1)
GSE72449 AhR links air pollution and atopic dermatitis via Artemin induction

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner -0.21191788
DarkCorner -0.15130806
A_52_P616356 -0.20558357
A_52_P580582 0.059447765
A_52_P403405 -0.3594017
A_52_P819156 0.3505187
A_51_P331831 0.29066396
A_51_P430630 -0.19130754
A_52_P502357 -0.1889453
A_52_P299964 0.084453106
A_51_P356389 0.2645638
A_52_P684402 0.06659651
A_51_P414208 0.032375336
A_51_P280918 -0.033111572
A_52_P613688 0.05012846
A_52_P258194 -0.23024082
A_52_P229271 -0.61834574
A_52_P214630 0.51422215
A_52_P579519 0.06381869
A_52_P979997 -0.16421747

Total number of rows: 41267

Table truncated, full table size 988 Kbytes.




Supplementary file Size Download File type/resource
GSM1862824_WT_Epidermis_Neonate_replicate2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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