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Sample GSM1862823 Query DataSets for GSM1862823
Status Public on Nov 13, 2016
Title WT_Epidermis_Neonate_replicate1
Sample type RNA
Source name WT, Epidermis, Neonate, replicate1
Organism Mus musculus
Characteristics genotype/variation: wild type
tissue: epidermis
strain: mix
age: postnatal day 0
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from epidermis using RNeasy Micro kit (Qiagen).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using the Quick Amp Labeling kit (Agilent) according to the manufacturer's protocol.
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Description Gene expression in wild-type epidermis at postnatal day 0, replicate1
Data processing The data were normalized by percentile shift, using GeneSpring GX software (Silicon Genetics).
Submission date Aug 27, 2015
Last update date Nov 13, 2016
Contact name Eri H Kobayashi
Organization name Tohoku University Graduate School of Medicine
Department Medical Biochemistry
Street address 2-1 Seiryo-machi, Aobaku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platform ID GPL7202
Series (1)
GSE72449 AhR links air pollution and atopic dermatitis via Artemin induction

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner -0.32812023
DarkCorner -0.1020875
A_52_P616356 -0.15424109
A_52_P580582 -0.3287573
A_52_P403405 -0.3096981
A_52_P819156 -0.14461946
A_51_P331831 1.0932939
A_51_P430630 -0.14335585
A_52_P502357 -0.14111471
A_52_P299964 0.47600412
A_51_P356389 -0.03133464
A_52_P684402 -0.041243076
A_51_P414208 -0.18510056
A_51_P280918 -0.49989414
A_52_P613688 0.13708687
A_52_P258194 -1.0903206
A_52_P229271 0.1115489
A_52_P214630 0.75745773
A_52_P579519 -0.06381869
A_52_P979997 -0.11337614

Total number of rows: 41267

Table truncated, full table size 991 Kbytes.

Supplementary file Size Download File type/resource
GSM1862823_WT_Epidermis_Neonate_replicate1.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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