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Sample GSM1861816 Query DataSets for GSM1861816
Status Public on Aug 27, 2015
Title HaCaT-1 [miRNA]
Sample type RNA
 
Source name HaCaT-1 [miRNA]
Organism Homo sapiens
Characteristics cell line: HaCaT
agent: none
Treatment protocol To prepare them for recombinant human IL-22 (Sangon Biological Engineering Technology Company, Shanghai, China) treatment, HaCaT cells were starved in serum-free DMEM for 24 h and then treated with IL-22 (100 ng/ml) in serum-free DMEM for another 24 h or not treated.
Growth protocol HaCaT cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS),100 U/ml penicillin and 100µg/ml streptomycin at 37℃ in a humidified atmosphere containing 5% CO2.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15ug total RNA
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 48C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip2 Scanner 3000 7G
Data processing GeneChip Command Console Software (AGCC).
 
Submission date Aug 26, 2015
Last update date Aug 27, 2015
Contact name Meng Jiang
E-mail(s) 1037447134@qq.com
Phone 15066142801
Organization name Qilu hospital
Street address lixia street
City Jinan
State/province Shandong
ZIP/Postal code 250012
Country China
 
Platform ID GPL19117
Series (1)
GSE72396 Expression data from HaCaT cells stimulated by IL-22

Data table header descriptions
ID_REF
VALUE rma-dabg-Signal
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
MIMAT0000062_st 13.16018 P
MIMAT0004481_st 0.5690693 A
MIMAT0010195_st 0.6669479 A
MIMAT0000063_st 13.66163 P
MIMAT0004482_st 2.287692 P
MIMAT0000064_st 12.90269 P
MIMAT0026472_st 0.9819524 A
MIMAT0000065_st 12.74877 P
MIMAT0004484_st 2.43577 P
MIMAT0000066_st 11.71704 P
MIMAT0004485_st 0.9514874 A
MIMAT0000067_st 9.99214 P
MIMAT0004486_st 1.870424 P
MIMAT0004487_st 0.4187538 A
MIMAT0000068_st 8.439442 P
MIMAT0004488_st 2.018988 P
MIMAT0000069_st 12.82158 P
MIMAT0004489_st 0.3881133 A
MIMAT0000070_st 12.43702 P
MIMAT0000071_st 6.418448 P

Total number of rows: 6658

Table truncated, full table size 177 Kbytes.




Supplementary file Size Download File type/resource
GSM1861816_duizhao-1_miRNA-4_0_.CEL.gz 770.3 Kb (ftp)(http) CEL
Processed data included within Sample table

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