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Sample GSM1858636 Query DataSets for GSM1858636
Status Public on Apr 08, 2016
Title untreated rep#2 ER ChIP-seq GH1008
Sample type SRA
 
Source name ZR-75-1 breast cancer cell line
Organism Homo sapiens
Characteristics cell line: ZR-75-1
cell type: Ascites from a ductal carcinoma of the breast
treatment: untreated
time: 30 minutes
replicate: replicate 2
antibody: ER cocktail: Ab-10 Thermo Scientific Lab Vision, HC-20 sc-543 Santa Cruz
growth proptocol: For maintenance cells were cultured in RPMI containing 2mM L-glutamine supplemented 10% calf serum, 1mM sodium pyruvate, 1X non-essential amino acids, and 1% penicillin-streptomycin
Treatment protocol Cells were plated for experiments in phenol red free growth medium supplemented with 10% charcoal-dextran serum twenty-four hours before hormone treatment. Cells were left untreated or induced with 100nM dexamethasone or 100nM estradiol for 30 minutes.
Growth protocol see additional columns of the SAMPLES section
Extracted molecule genomic DNA
Extraction protocol Lysates were clarified from sonicated nuclei and GR/DNA, ER/DNA, of FoxA1/DNA complexes were isolated with antibody.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
 
Description hs_RC_ZR751_ER_Unt_RC_GH999GH1008_FDR0.0.csv
Data processing RTA 1.12.42 was used for Basecalling and Casava 1.8.2 ws used for demultiplexing.
Alignment was performed by Bowtie2 software with the hg19 reference genome.
Areas of enrichment were identified using DNase2Hotspots software(http://sorceforge.net/proujects/dnase2hotspots/) software with FDR 0%.
Raw signal density graphs were generated by counting tags at each genomic coordinate after extending the length of sequences to 150bp by strands.
Values for tag densities were normalized to 10 million reads to adjust for differences in the sequencing depth between samples.
Genome_build: hg19
Supplementary_files_format_and_content: comma-delimited (CSV) text files include genomic locations (Chromosome, start, end ), z-score, p-value and the maximum density of hotspots. Bedgraph files contain genomic locations and normalized tag density profiles.
 
Submission date Aug 20, 2015
Last update date May 15, 2019
Contact name Songjoon Baek
Organization name NCI / NIH
Department CCR
Lab LRBGE
Street address 41 Library Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL11154
Series (2)
GSE72249 Characterization of ER, GR, and FoxA1 binding patterns in breast cancer cells treated with either Dex or E2
GSE72252 DNaseI hypersensitivity assay and ER, GR, and FoxA1 binding patterns in breast cancer cells
Relations
BioSample SAMN04004030
SRA SRX1161184

Supplementary file Size Download File type/resource
GSM1858636_GH1008_ZR751_ER_unt_ChIP_hg19.bedgraph.gz 60.8 Mb (ftp)(http) BEDGRAPH
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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