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Sample GSM1855890 Query DataSets for GSM1855890
Status Public on Aug 18, 2015
Title rat at C2, biological rep2
Sample type RNA
 
Source name Rats which were supplied with tap water for 2 weeks.
Organism Rattus norvegicus
Characteristics tissue: kidney
Treatment protocol experimental (T) group for the gene and protein expression studies and control (C) group. Rats in T group were provided free access to drinking water containing 1% (vol/vol) ethylene glycol and 0.5% ammonium chloride for a period of 2 weeks in order to induce hyperoxaluria, while the rats in C group were supplied with tap water for 2 weeks.
Growth protocol all rats were acclimated at 23℃ on a 12 h light/dark cycle before initiation of experiments in the SPF level animal house.
Extracted molecule total RNA
Extraction protocol total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol About 1ug total RNA were polyA tailed and labled with FlashTag™ Biotin HSR Labeling Kit(Affymetrix, Santa Clara, CA, USA).
 
Hybridization protocol The labled RNA were hybridized for 16 hr at 60 rpm at 48C on Affymetrix miRNA4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description Gene expression data from rats which were supplied with tap water for 2 weeks.
Data processing Raw data files were analyzed by using Affymetrix Expression Console with the default miRNA array settings. Raw data were processed and normalized on basis of organism rat using RMA-DABG algorithm.
 
Submission date Aug 18, 2015
Last update date Aug 18, 2015
Contact name Jiang Hongyang
E-mail(s) jiang.hongyang@163.com
Organization name Tongji Hospital, Huazhong University of Science and Technology
Street address No. 1095 Jie Fang Avenue
City Wuhan
State/province Hubei
ZIP/Postal code 430000
Country China
 
Platform ID GPL19117
Series (1)
GSE72135 Expression data from hyperoxaluric rats

Data table header descriptions
ID_REF
VALUE Log2 RMA signal
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
MIMAT0000001_st 13.81629 P
MIMAT0015091_st 0.2600741 A
MIMAT0000002_st 0.3797392 A
MIMAT0015092_st 0.458742 A
MIMAT0020301_st 0.2775294 A
MIMAT0000003_st 0.2126345 A
MIMAT0020302_st 0.8954566 P
MIMAT0000004_st 0.09096517 A
MIMAT0000005_st 3.302446 P
MIMAT0015093_st 1.226981 A
MIMAT0020303_st 0.2567087 A
MIMAT0000006_st 0.4069398 A
MIMAT0020304_st -0.001441807 A
MIMAT0000007_st 0.5470539 A
MIMAT0015094_st 0.3242105 A
MIMAT0000008_st 0.2101202 A
MIMAT0020305_st 0.1884228 A
MIMAT0000009_st 0.37643 A
MIMAT0020306_st -0.037788 A
MIMAT0000010_st 0.3963836 A

Total number of rows: 36249

Table truncated, full table size 974 Kbytes.




Supplementary file Size Download File type/resource
GSM1855890_C2_miRNA-4_0_.CEL.gz 780.6 Kb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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