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Sample GSM1855803 Query DataSets for GSM1855803
Status Public on Dec 31, 2016
Title HSF1 ZT14
Sample type SRA
Source name Liver
Organism Mus musculus
Characteristics strain: C57/BL6 WT
feeding: ad libitum
technique: ChIP-seq
tissue: liver
chip antibody: HSF1
time point: ZT 14
Extracted molecule genomic DNA
Extraction protocol For the HSF1 ChIP-Seq, chromatin preparation and chromatin immunoprecipitation were performed as in Rey et al., PLoS Biol 2011. The HSF1 polyclonal antibody is from Stressgen (Enzo Life Sciences). For each IP, 5ul of HSF1 antibody was used with 250ul of pre-cleared chromatin.
Libraries for ultra-high throughput sequencing were prepared with the ChIP-Seq DNA sample kit (Illumina) as recommended by the manufacturer.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2500
Description ZT 14
Data processing All alignments were performed using the BBCF HTSstation (available at, David et al., 2014)
Genome_build: mm9
Submission date Aug 17, 2015
Last update date May 04, 2020
Contact name Jonathan Aryeh Sobel
Organization name EPFL
Department School of Life Sciences
Lab Felix Naef Lab
Street address EPFL SV IBI-SV UPNAE AAB 0 36 (Batiment AAB) Station 15
City Lausanne
State/province Vaud
ZIP/Postal code CH-1015
Country Switzerland
Platform ID GPL17021
Series (2)
GSE60430 Regulatory logic of the coupled diurnal and feeding cycles in the mouse liver [DNase-seq, ChIP-seq]
GSE60578 Regulatory logic of the coupled diurnal and feeding cycles in the mouse liver
BioSample SAMN03997109
SRA SRX1155769

Supplementary file Size Download File type/resource 120.3 Mb (ftp)(http) BW
GSM1855803_HSF1_ZT14_macs2_narrowPeak_annotated.txt.gz 82.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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