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Sample GSM1841790 Query DataSets for GSM1841790
Status Public on Oct 01, 2015
Title 1122 2014 Lgr5 Ep- MIXED
Sample type RNA
 
Source name FACS sorted tumor cells
Organism Mus musculus
Characteristics genotype/variation: Lgr5CreER/KrasG12D/p53KO/Rosa-YFP
treatment: 10mg TAM
isolation: FACS
staining: Epcam-/Lin-/DAPI-
Extracted molecule total RNA
Extraction protocol qiagen kit
Label Biotin
Label protocol 25 ng total RNA was amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 8 µg of cDNA were subsequently fragmented and labeled using GeneChip Human Mapping 250K Nsp Assay Kit (Affymetrix; catalog # 900766), according to manufacturer's instructions.
 
Hybridization protocol cDNA was hybridized to Affymetrix Mouse Genome 430PM strip arrays for 16 hours at 45ºC.Washing and staining of microarrays was performed using the GeneAtlas Fluidics Station (Affymetrix, Santa Clara, CA)
Scan protocol GeneChips were scanned in a GeneAtlas Imaging Station (Affymetrix, Santa Clara, CA)
Data processing RMA normalization method
 
Submission date Jul 31, 2015
Last update date Oct 17, 2016
Contact name Cédric Blanpain
E-mail(s) cedric.blanpain@ulb.ac.be
Organization name ULB
Street address Route de Lennik 808
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL11180
Series (2)
GSE71621 Cell type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition
GSE87877 Cell type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition [expression 1]

Data table header descriptions
ID_REF
VALUE log2 RMA expression estimate

Data table
ID_REF VALUE
1415670_PM_at 10.3511
1415671_PM_at 10.1304
1415672_PM_at 7.42558
1415673_PM_at 8.83683
1415674_PM_a_at 7.39911
1415675_PM_at 8.50155
1415676_PM_a_at 10.8895
1415677_PM_at 10.1687
1415678_PM_at 9.68506
1415679_PM_at 10.8849
1415680_PM_at 8.57598
1415681_PM_at 8.44223
1415682_PM_at 6.80465
1415683_PM_at 7.46172
1415684_PM_at 3.39078
1415685_PM_at 6.2884
1415686_PM_at 8.58352
1415687_PM_a_at 10.5125
1415688_PM_at 7.23353
1415689_PM_s_at 5.80324

Total number of rows: 45141

Table truncated, full table size 983 Kbytes.




Supplementary file Size Download File type/resource
GSM1841790_1122_2014.ga_Lgr5_EP-_MIXED.cel.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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