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Sample GSM1841197 Query DataSets for GSM1841197
Status Public on Jul 31, 2017
Title B16F0 exosomes isolated from serum-free media media conditioned by B16F0 cells for 24 hr, rep 4
Sample type RNA
 
Source name B16F0 exosomes
Organism Mus musculus
Characteristics cell line: B16F0
sample type: exosomes isolated from B16F0 conditioned media
molecule subtype: exosomal RNA
Growth protocol Cultured for 24 hours in serum-free media
Extracted molecule total RNA
Extraction protocol Total RNA isolated from B16F0 exosomes and cells by RNeasy Plus kit (Qiagen) was quantified using Nanodrop and analyzed by on-chip-electrophoresis using the Agilent Bioanalyzer and was processed by the Ambion WT Expression Kit.
Label biotin
Label protocol Each reaction yielded between 6 to 9 micrograms of cDNA, whereby 5.5 mug of cDNA was fragmented and labeled with biotin by the GeneChip WT Terminal Labeling Kit (Affymetrix). The efficiency of fragmentation reaction was checked via Agilent Bioanalyzer.
 
Hybridization protocol The entire reaction of fragmented and biotin-labeled cDNA (50 mu) with added hybridization controls was hybridized to the mouse GeneChip 1.0 ST Exon Arrays (Affymetrix) at 45oC for 17 hours in GeneChip Hybridization Oven 640 (Affymetrix).
Scan protocol Mouse GeneChip 1.0 ST Exon Arrays were stained using FS 450_0001 protocol in Affymetrix GeneChip Fluidics Station 450. Phycoerythrin labeling was detected within the Affymetrix GeneChip Scanner 3000 7G plus using 532 nm light and detected by a photomultiplier tube.
Description 8 exosome 24h_(MoEx-1_0-st-v1)
Data processing Expression Console software (Affymetrix) was used to check quality controls of hybridized chips. All chips that passed quality controls were RMA normalized using Expression Console software.
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm8.mps
 
Submission date Jul 31, 2015
Last update date Jul 31, 2017
Contact name David John Klinke
E-mail(s) David.Klinke@mail.wvu.edu
Phone 304-293-9346
Organization name West Virginia University
Department Chemical Engineering
Street address P.O. Box 6102
City Morgantown
State/province WV
ZIP/Postal code 26506-6102
Country USA
 
Platform ID GPL6096
Series (2)
GSE71610 Expression data from parental B16F0 cells and B16F0 exosomes [MoEx]
GSE102951 Expression data from parental B16F0 cells and B16F0 exosomes

Data table header descriptions
ID_REF
VALUE RMA signal estimates from Expression Console Software.

Data table
ID_REF VALUE
6747308 7.396478
6747309 5.661499
6747314 6.501416
6747326 3.256782
6747343 4.799905
6747354 3.974601
6747364 6.938824
6747471 7.059421
6747472 9.45439
6747478 4.152571
6747497 6.725738
6747504 4.208636
6747515 5.383409
6747577 4.02514
6747641 4.695483
6747696 3.746166
6747786 5.238604
6747805 10.55386
6747837 6.109151
6747839 7.313551

Total number of rows: 23332

Table truncated, full table size 384 Kbytes.




Supplementary file Size Download File type/resource
GSM1841197_8_exosome_24h_MoEx-1_0-st-v1_.CEL.gz 20.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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