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Sample GSM1841196 Query DataSets for GSM1841196
Status Public on Jul 31, 2017
Title B16F0 exosomes isolated from serum-free media media conditioned by B16F0 cells for 24 hr, rep 3
Sample type RNA
Source name B16F0 exosomes
Organism Mus musculus
Characteristics cell line: B16F0
sample type: exosomes isolated from B16F0 conditioned media
molecule subtype: exosomal RNA
Growth protocol Cultured for 24 hours in serum-free media
Extracted molecule total RNA
Extraction protocol Total RNA isolated from B16F0 exosomes and cells by RNeasy Plus kit (Qiagen) was quantified using Nanodrop and analyzed by on-chip-electrophoresis using the Agilent Bioanalyzer and was processed by the Ambion WT Expression Kit.
Label biotin
Label protocol Each reaction yielded between 6 to 9 micrograms of cDNA, whereby 5.5 mug of cDNA was fragmented and labeled with biotin by the GeneChip WT Terminal Labeling Kit (Affymetrix). The efficiency of fragmentation reaction was checked via Agilent Bioanalyzer.
Hybridization protocol The entire reaction of fragmented and biotin-labeled cDNA (50 mu) with added hybridization controls was hybridized to the mouse GeneChip 1.0 ST Exon Arrays (Affymetrix) at 45oC for 17 hours in GeneChip Hybridization Oven 640 (Affymetrix).
Scan protocol Mouse GeneChip 1.0 ST Exon Arrays were stained using FS 450_0001 protocol in Affymetrix GeneChip Fluidics Station 450. Phycoerythrin labeling was detected within the Affymetrix GeneChip Scanner 3000 7G plus using 532 nm light and detected by a photomultiplier tube.
Description 7 exosome 24h_(MoEx-1_0-st-v1)
Data processing Expression Console software (Affymetrix) was used to check quality controls of hybridized chips. All chips that passed quality controls were RMA normalized using Expression Console software.
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm8.mps
Submission date Jul 31, 2015
Last update date Jul 31, 2017
Contact name David John Klinke
Phone 304-293-9346
Organization name West Virginia University
Department Chemical Engineering
Street address P.O. Box 6102
City Morgantown
State/province WV
ZIP/Postal code 26506-6102
Country USA
Platform ID GPL6096
Series (2)
GSE71610 Expression data from parental B16F0 cells and B16F0 exosomes [MoEx]
GSE102951 Expression data from parental B16F0 cells and B16F0 exosomes

Data table header descriptions
VALUE RMA signal estimates from Expression Console Software.

Data table
6747308 7.477351
6747309 6.031204
6747314 6.474751
6747326 3.752801
6747343 4.859885
6747354 3.850057
6747364 6.976085
6747471 7.644876
6747472 9.242125
6747478 4.660683
6747497 6.957246
6747504 4.011686
6747515 5.157196
6747577 4.067404
6747641 4.452414
6747696 3.615959
6747786 5.337845
6747805 10.54025
6747837 5.894581
6747839 7.40518

Total number of rows: 23332

Table truncated, full table size 384 Kbytes.

Supplementary file Size Download File type/resource
GSM1841196_7_exosome_24h_MoEx-1_0-st-v1_.CEL.gz 21.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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