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Sample GSM1841190 Query DataSets for GSM1841190
Status Public on Jul 31, 2017
Title B16F0 cells cultured for 24 hr in serum-free media, rep 1
Sample type RNA
 
Source name B16F0 cells
Organism Mus musculus
Characteristics cell line: B16F0
sample type: parental B16F0 cells
molecule subtype: total RNA
Growth protocol Cultured for 24 hours in serum-free media
Extracted molecule total RNA
Extraction protocol Total RNA isolated from B16F0 exosomes and cells by RNeasy Plus kit (Qiagen) was quantified using Nanodrop and analyzed by on-chip-electrophoresis using the Agilent Bioanalyzer and was processed by the Ambion WT Expression Kit.
Label biotin
Label protocol Each reaction yielded between 6 to 9 micrograms of cDNA, whereby 5.5 mug of cDNA was fragmented and labeled with biotin by the GeneChip WT Terminal Labeling Kit (Affymetrix). The efficiency of fragmentation reaction was checked via Agilent Bioanalyzer.
 
Hybridization protocol The entire reaction of fragmented and biotin-labeled cDNA (50 mu) with added hybridization controls was hybridized to the mouse GeneChip 1.0 ST Exon Arrays (Affymetrix) at 45oC for 17 hours in GeneChip Hybridization Oven 640 (Affymetrix).
Scan protocol Mouse GeneChip 1.0 ST Exon Arrays were stained using FS 450_0001 protocol in Affymetrix GeneChip Fluidics Station 450. Phycoerythrin labeling was detected within the Affymetrix GeneChip Scanner 3000 7G plus using 532 nm light and detected by a photomultiplier tube.
Description 1 cell 24h_(MoEx-1_0-st-v1)
Data processing Expression Console software (Affymetrix) was used to check quality controls of hybridized chips. All chips that passed quality controls were RMA normalized using Expression Console software.
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm8.mps
 
Submission date Jul 31, 2015
Last update date Jul 31, 2017
Contact name David John Klinke
E-mail(s) David.Klinke@mail.wvu.edu
Phone 304-293-9346
Organization name West Virginia University
Department Chemical Engineering
Street address P.O. Box 6102
City Morgantown
State/province WV
ZIP/Postal code 26506-6102
Country USA
 
Platform ID GPL6096
Series (2)
GSE71610 Expression data from parental B16F0 cells and B16F0 exosomes [MoEx]
GSE102951 Expression data from parental B16F0 cells and B16F0 exosomes

Data table header descriptions
ID_REF
VALUE RMA signal estimates from Expression Console Software.

Data table
ID_REF VALUE
6747308 10.02728
6747309 10.07514
6747314 9.689451
6747326 4.548631
6747343 7.35597
6747354 3.568519
6747364 8.616496
6747471 7.895607
6747472 9.95126
6747478 4.082648
6747497 8.683412
6747504 3.64439
6747515 7.288437
6747577 3.96356
6747641 4.369592
6747696 3.586987
6747786 7.904092
6747805 7.506361
6747837 8.135743
6747839 10.32098

Total number of rows: 23332

Table truncated, full table size 384 Kbytes.




Supplementary file Size Download File type/resource
GSM1841190_1_cell_24h_MoEx-1_0-st-v1_.CEL.gz 23.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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