|
| Status |
Public on Jul 31, 2015 |
| Title |
Tamoxifen 12h |
| Sample type |
RNA |
| |
|
| Source name |
Gastric Corpus Epithelium
|
| Organism |
Mus musculus |
| Characteristics |
genotype: WT
agent: Tamoxifen
time point: 12 hours
|
| Treatment protocol |
Tamoxifen (5mg/20g body weight) was injected intraperitoneally for one or three days to induce SPEM and mice were dissected at 12 hours, 2.5 days, and 4.5 days after treatment. Tamoxifen was dissolved in a vehicle of 10% ethanol and 90% sunflower seed oil (Sigma)
|
| Growth protocol |
All experiments involving animals were performed according to protocols approved by the Washington University School of Medicine Animal Studies Committee. Mice were maintained in a specified-pathogen-free barrier facility under a 12 hour light cycle. Wild type C57BL/6, BALB/c and FVB/N mice were purchased from The Jackson Laboratory. Mice were 6 weeks old at time of treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted, purified by RNEasy Mini Kit, and RNA integrity verified on Agilent Bioanalyzer
|
| Label |
biotin
|
| Label protocol |
Total RNA extracted and pooled from 3 dissections and mice was amplirifed, labeled, and fragmented (by Arctureus RiboAmp HS kit followed by the RNA Amplification and Labeling Kit from Enzo Life Sciences)
|
| |
|
| Hybridization protocol |
Total RNA was hybridized to Affymetrix GeneChip Mouse Gene 2.0 ST Arrays according to manufacturers instructions
|
| Scan protocol |
Arrays were scanned with a GeneChip® Scanner 3000 laser confocal slide scanner
|
| Data processing |
Microarray data were analyzed using Partek Genomic Suite v6.6 (Partek Inc., St Louis, MO). Microarray CEL files were imported into Partek and normalized using RMA.
|
| |
|
| Submission date |
Jul 30, 2015 |
| Last update date |
Jul 31, 2015 |
| Contact name |
Jason Mills |
| E-mail(s) |
jmills@wustl.edu
|
| Phone |
3143624258
|
| Organization name |
Washington University in St. Louis
|
| Street address |
Department of Medicine - GI
|
| City |
St. Louis |
| State/province |
Mo |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE71580 |
High Dose Tamoxifen in the Mouse Gastric Epithelium |
|
