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Sample GSM1835021 Query DataSets for GSM1835021
Status Public on Mar 16, 2016
Title pES10 NPC h-G1
Sample type SRA
Source name Haploid NCAM1+ pES10-NPCs in G1 (1c)
Organism Homo sapiens
Characteristics cell line: h-pES10
cell type: Haploid NCAM1+ pES10-NPCs
cell cycle phase: G1 (1c)
passage: 21
differentiation status: Differentiated
Extracted molecule polyA RNA
Extraction protocol RNA was isolated using the RNeasy Micro Kit or the RNeasy Mini Kit (Qiagen). For RNA sequencing analysis, poly(A) pull-down was used to enrich mRNAs from total RNA samples (200 ng–1 µg per sample)
Libraries were prepared using the Illumina TruSeq RNA prep kit v2
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Description Haploid NCAM1+ NPCs in G1 (1c) derived from ES cells after 4th sort + 10 days culture + 10 days differentiation
Data processing Reads were mapped to the human reference genome (GRCh37/hg19) using TopHat (version 2.0.8b)
Reads per kilobase of transcript per million mapped reads (RPKM) values were calculated using Cufflinks (version 2.1.1)
Genome_build: Ensembl/GRCh37/hg19
Supplementary_files_format_and_content: Tab-delimited text file includes Abundance measurements (RPKM) for each sample
Submission date Jul 28, 2015
Last update date May 15, 2019
Contact name Ido Sagi
Organization name The Hebrew University of Jerusalem
Department Genetics
Lab Nissim Benvenisty Lab, The Azrieli Center for Stem Cells and Genetic Research
Street address Givat Ram
City Jerusalem
ZIP/Postal code 91904
Country Israel
Platform ID GPL18573
Series (2)
GSE71456 Derivation and differentiation of haploid human embryonic stem cells [RNA-Seq 1]
GSE71458 Derivation and differentiation of haploid human embryonic stem cells
BioSample SAMN03940962
SRA SRX1122437

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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