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Sample GSM1835018 Query DataSets for GSM1835018
Status Public on Mar 16, 2016
Title pES12 h-G1 rep2
Sample type SRA
 
Source name Haploid pES12 cells in G1 (1c) rep2
Organism Homo sapiens
Characteristics cell line: h-pES12
cell type: Haploid pES12 cells
cell cycle phase: G1 (1c)
passage: 22
differentiation status: Undifferentiated
Extracted molecule polyA RNA
Extraction protocol RNA was isolated using the RNeasy Micro Kit or the RNeasy Mini Kit (Qiagen). For RNA sequencing analysis, poly(A) pull-down was used to enrich mRNAs from total RNA samples (200 ng–1 µg per sample)
Libraries were prepared using the Illumina TruSeq RNA prep kit v2
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description Haploid ES cells in G1 (1c), 5th sort, biological replicate 2
Data processing Reads were mapped to the human reference genome (GRCh37/hg19) using TopHat (version 2.0.8b)
Reads per kilobase of transcript per million mapped reads (RPKM) values were calculated using Cufflinks (version 2.1.1)
Genome_build: Ensembl/GRCh37/hg19
Supplementary_files_format_and_content: Tab-delimited text file includes Abundance measurements (RPKM) for each sample
 
Submission date Jul 28, 2015
Last update date May 15, 2019
Contact name Ido Sagi
Organization name The Hebrew University of Jerusalem
Department Genetics
Lab Nissim Benvenisty Lab, The Azrieli Center for Stem Cells and Genetic Research
Street address Givat Ram
City Jerusalem
ZIP/Postal code 91904
Country Israel
 
Platform ID GPL18573
Series (2)
GSE71456 Derivation and differentiation of haploid human embryonic stem cells [RNA-Seq 1]
GSE71458 Derivation and differentiation of haploid human embryonic stem cells
Relations
BioSample SAMN03940959
SRA SRX1122434

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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