|
| Status |
Public on Nov 20, 2008 |
| Title |
IGF-treated cells (24hr), biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7
|
| Organism |
Homo sapiens |
| Characteristics |
IGF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was carried out using EZ1 RNA universal tissue kit with BioRobot EZ1 workstation according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized on Human Genome U133 A_2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-ws2v5 450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner (Affymetrix).
|
| Description |
cells from primary breast cancers were flow sorted to select for CD44+/CD24- cells and compare with the rest of the cells
|
| Data processing |
The data were analyzed with dChip version2006 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10%. of intensity values
|
| |
|
| Submission date |
Apr 20, 2007 |
| Last update date |
Jan 24, 2011 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE7561 |
Expression data from IGF-I-stimulated MCF-7 cells |
|
| Relations |
| Reanalyzed by |
GSM659821 |
