|
| Status |
Public on Jul 29, 2015 |
| Title |
Early Blight Resistant Control-1 |
| Sample type |
RNA |
| |
|
| Source name |
Resistant plant leaf at vegetative stage in normal growth condition
|
| Organism |
Solanum habrochaites |
| Characteristics |
genotype: Early Blight Tolerant Genotype
tissue: leaf
treatment: control
|
| Treatment protocol |
Sample 1,2,3,7,8,9 were grown in normal growth conditions without any stress in green house. While sample 4,5,6, and 10,11,12 were challenged with 10 days old culture A. solani in optimal conditions for disease development in chamber . The sample was collected after 24hrs of inoculation at which fungal mycelia start to penetrate the host tissue (Dita et al. 2007)
|
| Growth protocol |
Plants were sown in plastic pots at optimal conditions for tomato plants.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Tomato Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
|
| Description |
Gene expression data from Tomato Early Blight Resistant untreated plants
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jul 28, 2015 |
| Last update date |
Jul 29, 2015 |
| Contact name |
PRITI UPADHYAY |
| E-mail(s) |
priti.iasbhu@gmail.com
|
| Organization name |
BANARAS HINDU UNIVERSITY
|
| Department |
GENETICS AND PLANT BREEDING
|
| Street address |
VARANASI
|
| City |
VARANASI |
| ZIP/Postal code |
221005 |
| Country |
India |
| |
|
| Platform ID |
GPL4741 |
| Series (1) |
| GSE71428 |
Microarray Expression profiling of susceptible (CO-3) and resistant (EC-520061) genotype of tomato in response to Early Blight pathogen after 24hrs of inoculation |
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