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Sample GSM1831614 Query DataSets for GSM1831614
Status Public on May 12, 2016
Title M2-BMDMs_WT_replicate2
Sample type RNA
 
Source name BMDMs, WT, IL-4, 6hrs, replicate2
Organism Mus musculus
Characteristics strain: C57BL/6
gender: Female
age: 8 weeks
cell type: BMDM
genotype/variation: WT
treatment: M2-stimuli (IL-4)
Treatment protocol BMDMs are untreated, or treated with M1-stimuli (LPS and IFNg) or with M2-stimuli (IL-4) for 6 hours.
Growth protocol Bone marrow cells were cultured in DMEM supplemented with 10% fetal bovine serum, M-CSF, and Antibiotic-Antimycotic. The cultures were incubated at 37 ÂșC in a humidified incubator with 5% CO2 for 7 days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from BMDMs using RNeasy Micro kit (Qiagen).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using the Quick Amp Labeling kit (Agilent) according to the manufacturer's protocol.
 
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Description Gene expression in wild-type BMDMs stimulated with IL-4 for 6hrs, replicate2
Data processing The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
 
Submission date Jul 23, 2015
Last update date May 12, 2016
Contact name Eri H Kobayashi
Organization name Tohoku University Graduate School of Medicine
Department Medical Biochemistry
Street address 2-1 Seiryo-machi, Aobaku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
 
Platform ID GPL7202
Series (1)
GSE71263 Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner -0.06059742
DarkCorner -0.036509514
A_52_P616356 0.6323385
A_52_P580582 -0.16620159
A_52_P403405 -0.8515363
A_52_P819156 0.19093513
A_51_P331831 -0.32782602
A_51_P430630 -0.021943092
A_52_P502357 -0.021882057
A_52_P299964 -0.9675255
A_51_P356389 -0.15881252
A_52_P684402 0.2646613
A_51_P414208 -0.021659851
A_51_P280918 -0.08649635
A_52_P613688 -1.2295704
A_52_P258194 -0.24147558
A_52_P229271 -0.14310455
A_52_P214630 0.24675941
A_52_P579519 1.2623305
A_52_P979997 -0.021391869

Total number of rows: 40842

Table truncated, full table size 975 Kbytes.




Supplementary file Size Download File type/resource
GSM1831614_M2-BMDMs_WT_replicate2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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