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Sample GSM1831613 Query DataSets for GSM1831613
Status Public on May 12, 2016
Title M2-BMDMs_WT_replicate1
Sample type RNA
 
Source name BMDMs, WT, IL-4, 6hrs, replicate1
Organism Mus musculus
Characteristics strain: C57BL/6
gender: Male
age: 8 weeks
cell type: BMDM
genotype/variation: WT
treatment: M2-stimuli (IL-4)
Treatment protocol BMDMs are untreated, or treated with M1-stimuli (LPS and IFNg) or with M2-stimuli (IL-4) for 6 hours.
Growth protocol Bone marrow cells were cultured in DMEM supplemented with 10% fetal bovine serum, M-CSF, and Antibiotic-Antimycotic. The cultures were incubated at 37 ÂșC in a humidified incubator with 5% CO2 for 7 days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from BMDMs using RNeasy Micro kit (Qiagen).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using the Quick Amp Labeling kit (Agilent) according to the manufacturer's protocol.
 
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Description Gene expression in wild-type BMDMs stimulated with IL-4 for 6hrs, replicate1
Data processing The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
 
Submission date Jul 23, 2015
Last update date May 12, 2016
Contact name Eri H Kobayashi
Organization name Tohoku University Graduate School of Medicine
Department Medical Biochemistry
Street address 2-1 Seiryo-machi, Aobaku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
 
Platform ID GPL7202
Series (1)
GSE71263 Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 0.47821617
DarkCorner 0.1349659
A_52_P616356 -0.30688953
A_52_P580582 0.020431519
A_52_P403405 -0.076282024
A_52_P819156 0.14206743
A_51_P331831 -0.30368185
A_51_P430630 0.2762394
A_52_P502357 0.27698135
A_52_P299964 0.5201607
A_51_P356389 0.008100987
A_52_P684402 0.04051733
A_51_P414208 0.2758627
A_51_P280918 0.055745125
A_52_P613688 -0.8723297
A_52_P258194 -0.31075191
A_52_P229271 -0.105072975
A_52_P214630 -0.17993641
A_52_P579519 2.209784
A_52_P979997 0.26186943

Total number of rows: 40842

Table truncated, full table size 968 Kbytes.




Supplementary file Size Download File type/resource
GSM1831613_M2-BMDMs_WT_replicate1.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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