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Sample GSM1831612 Query DataSets for GSM1831612
Status Public on May 12, 2016
Title M1-BMDMs_Keap1CKO_replicate2
Sample type RNA
Source name BMDMs, Keap1CKO, LPS and IFNg, 6hrs, replicate2
Organism Mus musculus
Characteristics strain: C57BL/6
gender: Female
age: 8 weeks
cell type: BMDM
genotype/variation: Keap1 CKO
treatment: M1-stimuli (LPS and IFNg)
Treatment protocol BMDMs are untreated, or treated with M1-stimuli (LPS and IFNg) or with M2-stimuli (IL-4) for 6 hours.
Growth protocol Bone marrow cells were cultured in DMEM supplemented with 10% fetal bovine serum, M-CSF, and Antibiotic-Antimycotic. The cultures were incubated at 37 ºC in a humidified incubator with 5% CO2 for 7 days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from BMDMs using RNeasy Micro kit (Qiagen).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using the Quick Amp Labeling kit (Agilent) according to the manufacturer's protocol.
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Description Gene expression in Keap1-deficient BMDMs stimulated with LPS and IFNg for 6hrs, replicate2
Data processing The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
Submission date Jul 23, 2015
Last update date May 12, 2016
Contact name Eri H Kobayashi
Organization name Tohoku University Graduate School of Medicine
Department Medical Biochemistry
Street address 2-1 Seiryo-machi, Aobaku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platform ID GPL7202
Series (1)
GSE71263 Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.06059742
DarkCorner 0.39216328
A_52_P616356 -0.94668293
A_52_P580582 0.7545867
A_52_P403405 -0.4124384
A_52_P819156 -1.2257686
A_51_P331831 1.1143479
A_51_P430630 4.9158554
A_52_P502357 0.43717766
A_52_P299964 -0.1148386
A_51_P356389 -0.27728415
A_52_P684402 -0.42305517
A_51_P414208 0.4449501
A_51_P280918 -0.092707634
A_52_P613688 0.22496605
A_52_P258194 -0.08569431
A_52_P229271 1.1413398
A_52_P214630 -0.65371037
A_52_P579519 -4.0965266
A_52_P979997 0.45064354

Total number of rows: 40842

Table truncated, full table size 957 Kbytes.

Supplementary file Size Download File type/resource
GSM1831612_M1-BMDMs_KpCKO_replicate2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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