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Sample GSM1831609 Query DataSets for GSM1831609
Status Public on May 12, 2016
Title M1-BMDMs_WT_replicate1
Sample type RNA
Source name BMDMs, WT, LPS and IFNg, 6hrs, replicate1
Organism Mus musculus
Characteristics strain: C57BL/6
gender: Male
age: 8 weeks
cell type: BMDM
genotype/variation: WT
treatment: M1-stimuli (LPS and IFNg)
Treatment protocol BMDMs are untreated, or treated with M1-stimuli (LPS and IFNg) or with M2-stimuli (IL-4) for 6 hours.
Growth protocol Bone marrow cells were cultured in DMEM supplemented with 10% fetal bovine serum, M-CSF, and Antibiotic-Antimycotic. The cultures were incubated at 37 ÂșC in a humidified incubator with 5% CO2 for 7 days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from BMDMs using RNeasy Micro kit (Qiagen).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using the Quick Amp Labeling kit (Agilent) according to the manufacturer's protocol.
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Description Gene expression in wild-type BMDMs stimulated with LPS and IFNg for 6hrs, replicate1
Data processing The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
Submission date Jul 23, 2015
Last update date May 12, 2016
Contact name Eri H Kobayashi
Organization name Tohoku University Graduate School of Medicine
Department Medical Biochemistry
Street address 2-1 Seiryo-machi, Aobaku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platform ID GPL7202
Series (1)
GSE71263 Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 1.5444956
DarkCorner 1.0953865
A_52_P616356 -0.16153336
A_52_P580582 1.4990773
A_52_P403405 0.9023428
A_52_P819156 -2.2569127
A_51_P331831 -0.19131804
A_51_P430630 5.6818013
A_52_P502357 1.2076416
A_52_P299964 0.68192816
A_51_P356389 -0.117602825
A_52_P684402 -0.74373007
A_51_P414208 1.2021556
A_51_P280918 -0.28146172
A_52_P613688 0.44878483
A_52_P258194 0.4837761
A_52_P229271 1.3085375
A_52_P214630 -0.5150175
A_52_P579519 -3.852735
A_52_P979997 1.1800871

Total number of rows: 40842

Table truncated, full table size 946 Kbytes.

Supplementary file Size Download File type/resource
GSM1831609_M1-BMDMs_WT_replicate1.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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