|
| Status |
Public on Aug 22, 2016 |
| Title |
im_S_XCR1+_LT_SetD_1 |
| Sample type |
RNA |
| |
|
| Source name |
immature (total) spleen XCR1+ DC
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
phenotype: Lin-CD11chighCD11b-CD8α+
cell type: immature (total) spleen XCR1+ DC
treatment: Untreated
|
| Treatment protocol |
Mice were untreated or infected for 1.5 days i.p. with 2.5x10E4 pfu of salivary-gland extracted MCMV Smith strain.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Each cell subset was purified by flow cytometry and total RNA was extracted with the Qiagen RNeasy Plus Micro Kit, according to the manufacturer protocol.
|
| Label |
biotin
|
| Label protocol |
20 ng of RNA from each sample was used to synthesize biotinylated probes, using 2 successive rounds of cRNA amplification (using the NuGEN Ovation Pico WTA System V2 Kit and the NuGEN Encore Biotin Module Kit according to NuGEN recommendations) with appropriate quality control to ensure full length synthesis.
|
| |
|
| Hybridization protocol |
Following fragmentation and end-labeling, 2 μg of cDNAs were hybridized for 16 hours at 45°C on Affymetrix GeneChip Mouse Gene 1.0 ST arrays. The chips were washed and stained in the GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G (Affymetrix) at a resolution of 0.7 µm.
|
| Data processing |
The data were RMA normalized in R and Bioconductor, using the oligo package.
|
| |
|
| Submission date |
Jul 21, 2015 |
| Last update date |
Oct 26, 2018 |
| Contact name |
Marc DALOD |
| E-mail(s) |
dalod@ciml.univ-mrs.fr
|
| Organization name |
CIML
|
| Street address |
Parc Scientifique et Technologique de Luminy, 163 avenue de Luminy, Case 906, 13288 Marseille cedex 9
|
| City |
MARSEILLE |
| ZIP/Postal code |
13009 |
| Country |
France |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE71169 |
Gene expression profiling of splenic XCR1+ dendritic cells from untreated or day 1.5 MCMV-infected mice |
| GSE71171 |
Broad and largely overlapping molecular changes arise in thymic and peripheral XCR1+ dendritic cells upon tolerogenic and immunogenic maturation |
|
| Relations |
| Reanalyzed by |
GSE115450 |
| Reanalyzed by |
GSE121859 |
