|
Status |
Public on Aug 31, 2016 |
Title |
B_50741_92 |
Sample type |
RNA |
|
|
Source name |
benign
|
Organism |
Homo sapiens |
Characteristics |
gleason_score: 4+3 ethnicity: African-American
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted following the manufacturer's recommendations.
|
Label |
cy3
|
Label protocol |
The cRNA labeling was performed according to Protocol from Agilent Technologies Inc. (Santa Clara, CA) using the Agilent Quick Amp Labeling Kit (Agilent Technologies, Palo Alto, CA) following the two-colour manufacturer's protocol.
|
|
|
Hybridization protocol |
cRNA samples were hybridized to an Agilent Technologies Human Gene Expression 8x60K Microarray Agilent Technologies). The hybridization slides were washed, stabilized, dried, and immediately scanned.
|
Scan protocol |
Scanned with an Agilent Technologies Microarray Scanner using default parameters. Each sample represents one channel of a dual-channel array.
|
Data processing |
Expression arrays were processed using BioConductor (one color arrays, Quantile normalization). Also we combated the dataset by patient (as the “batch” variable).
|
|
|
Submission date |
Jul 16, 2015 |
Last update date |
Apr 23, 2018 |
Contact name |
Chad Creighton |
E-mail(s) |
creighto@bcm.tmc.edu
|
Organization name |
Baylor College of Medicine
|
Department |
Biostatistics, Ducan Cancer Center
|
Street address |
One Baylor Plaza, Mail Stop: BCM305
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL16699 |
Series (1) |
GSE71016 |
Gene expression profiling of African-American prostate cancer |
|
Relations |
Reanalyzed by |
GSE113533 |