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Sample GSM1812079 Query DataSets for GSM1812079
Status Public on Mar 21, 2016
Title FTC236 Notch1_48hr_1ugDox_rep2
Sample type RNA
Source name FTC236-Notch1 cell line, 48hr, 1ug/ml Dox, replicate 2
Organism Homo sapiens
Characteristics type of cancer: follicular cancer
target gene turned on: Yes
tissue: thyroid
cell line: FTC236
Treatment protocol FTC236-Notch1 cells were treated with doxycycline at 1 µg/mL or without doxycycline.
Growth protocol FTC236-Notch1 cells were maintained in DMEM/Ham’s F-12 (1:1; Invitrogen) medium supplemented with 10% FBS, 0.01 U/mL of thyroid-stimulating hormone (TSH) and 10 µg/mL of insulin (Sigma-Aldrich) and were under dual antibiotic selection (G418 and hygromycin)
Extracted molecule total RNA
Extraction protocol Processed with an Qiagen Rneasy Mini kit
Label Cy3
Label protocol Total RNA was amplified and labeled according to manufactures instructions. Agilent One Color Microarray-Based Gene Expression Analysis Agilent low RNA input linear amplification kit, PLUS
Hybridization protocol Agilent One-Color Microarray-Based Gene Expression Analysis 17hrs 65 deg celcius Surehyb chamber
Scan protocol Agilent Technologies Scanner G2505B US22502567 3um 16bit
Data processing Agilent Feature extraction version for background subtraction - Background and Multiplicitive detrending. Data analyzed in R using Limma. Threshold: 1.0 Logbase: 2 Technology: Agilent.SingleColor.28005 Normalization: Quantile
Submission date Jul 08, 2015
Last update date Mar 21, 2016
Contact name Brian P Johnson
Phone 608-262-1209
Organization name UW-Madison
Department Oncology
Lab Chris Bradfield
Street address 1400 University Ave
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
Platform ID GPL13607
Series (1)
GSE70627 Notch1 Signaling Regulates the Aggressiveness of Differentiated Thyroid Cancer and Inhibits SERPINE1 Expression

Data table header descriptions
VALUE Quantile normalized log2data.

Data table
1 15.23146701
2 5.810902363
3 5.943793232
4 10.97200177
5 10.94484347
6 7.909720317
7 14.89103469
8 10.08792183
9 6.56561547
10 7.64124159
11 6.64344065
12 9.8559267
13 9.557966916
14 8.853479657
15 14.3930924
16 6.307814669
17 10.79107159
18 8.870275939
19 6.491391378
20 11.0961471

Total number of rows: 62976

Table truncated, full table size 1089 Kbytes.

Supplementary file Size Download File type/resource
GSM1812079_US10130359_252800415523_S01_GE1_107_Sep09_1_4.txt.gz 12.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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