|
| Status |
Public on Mar 21, 2016 |
| Title |
FTC236 Notch1_48hr_1ugDox_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
FTC236-Notch1 cell line, 48hr, 1ug/ml Dox, replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
type of cancer: follicular cancer
target gene turned on: Yes
tissue: thyroid
cell line: FTC236
|
| Treatment protocol |
FTC236-Notch1 cells were treated with doxycycline at 1 µg/mL or without doxycycline.
|
| Growth protocol |
FTC236-Notch1 cells were maintained in DMEM/Ham’s F-12 (1:1; Invitrogen) medium supplemented with 10% FBS, 0.01 U/mL of thyroid-stimulating hormone (TSH) and 10 µg/mL of insulin (Sigma-Aldrich) and were under dual antibiotic selection (G418 and hygromycin)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Processed with an Qiagen Rneasy Mini kit
|
| Label |
Cy3
|
| Label protocol |
Total RNA was amplified and labeled according to manufactures instructions. Agilent One Color Microarray-Based Gene Expression Analysis Agilent low RNA input linear amplification kit, PLUS
|
| |
|
| Hybridization protocol |
Agilent One-Color Microarray-Based Gene Expression Analysis 17hrs 65 deg celcius Surehyb chamber
|
| Scan protocol |
Agilent Technologies Scanner G2505B US22502567 3um 16bit
|
| Data processing |
Agilent Feature extraction version 10.7.3.1 for background subtraction - Background and Multiplicitive detrending. Data analyzed in R using Limma. Threshold: 1.0 Logbase: 2 Technology: Agilent.SingleColor.28005 Normalization: Quantile
|
| |
|
| Submission date |
Jul 08, 2015 |
| Last update date |
Mar 21, 2016 |
| Contact name |
Brian P Johnson |
| Phone |
608-262-1209
|
| Organization name |
UW-Madison
|
| Department |
Oncology
|
| Lab |
Chris Bradfield
|
| Street address |
1400 University Ave
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE70627 |
Notch1 Signaling Regulates the Aggressiveness of Differentiated Thyroid Cancer and Inhibits SERPINE1 Expression |
|
