|
| Status |
Public on Apr 01, 2016 |
| Title |
time point 40 experiment 2 arrested cells |
| Sample type |
SRA |
| |
|
| Source name |
time point 40 arrested cells
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
protocol: arrested
time: 40 min
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hot phenol RNA extraction
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
40A exp2
|
| Data processing |
Mapping with Tophat to S.pombe reference genome, with default parameters, tophat/2.0.8b
Counts generated with htseq, htseq/0.5.3p3
Differential expression analysis performed with DESeq2
Genome_build: EF2 (ASM294v2)
Supplementary_files_format_and_content: tab-delimited text file including raw counts for each sample
|
| |
|
| Submission date |
Jul 01, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Zsolt Szilagyi |
| Organization name |
University of Gothenburg
|
| Street address |
Medicinaregatan 9A
|
| City |
Gothenburg |
| ZIP/Postal code |
41390 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL13988 |
| Series (2) |
| GSE70435 |
RNAseq measurments of gene expression during simultanous replication and mitosis using the S. Pombe minimal cell cycle strain |
| GSE70717 |
Cdk1 activity levels drive periodic transcription independently of cell cycle progression |
|
| Relations |
| BioSample |
SAMN03835459 |
| SRA |
SRX1078767 |
