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Sample GSM1754619 Query DataSets for GSM1754619
Status Public on Aug 22, 2015
Title TB015N
Sample type RNA
Source name Cell specific microRNA transcriptome from blood granulocytes isolated from a household contact, male
Organism Homo sapiens
Characteristics tissue: granulocytes
gender: male
study group: household contact
matched pair id: P17
subject id: TB015
Treatment protocol Patients presenting with TB symptoms (TB, n=8) were recruited on the day of diagnosis, and subsequently, LTBI participants (LTBI, n=8) were recruited to match the patients' age and gender. Granulocytes and monocytes were sequentially separated from peripheral blood by magnetic beads (MACS, Miltenyi Biotec GmbH, CD15+ and CD14+, respectively) according to manufacturer's instructions.
Extracted molecule total RNA
Extraction protocol Total RNA (tRNA) was isolated using TRIzol® reagent (Life Technologies Corporation) according to manufacturer's instructions. Quality and quantisty of nucleic acids were determined by electrophoresis and spectrophotometry.
Label Cy3
Label protocol Total RNA was labeled with the single-color Quick amp Labeling Kit (Agilent Technologies) according to manufacturer’s instructions.
Hybridization protocol After precipitation, purification and quantification of the labeling reactions, 1.25µg labeled samples were hybridized according to the supplier's protocol (Agilent Technologies). Washing of the hybridizations was done with the SSPE wash protocol according to the supplier's protocol (Agilent Technologies).
Scan protocol Scanning of microarrays was performed with 5µm resolution and extended range using a G2565CA high resolution laser microarray scanner (Agilent Technologies).
Description Cell specific microRNA transcriptome from blood granulocytes isolated from a household contact, male
Data processing Microarray image data were extracted and analyzed with the Image Analysis / Feature Extraction software G2567AA (Version A., Agilent Technologies) using the protocol GE1_1100_Jul11 according the supplier's recommendations. The raw intensity files were read into limma / BioConductor, background corrected using the normexp method and normalized using the quantile method. Intensities were averaged for identical probes and control probes were removed.
Submission date Jul 01, 2015
Last update date Jan 06, 2023
Contact name January 3rd Weiner
Phone 030450543049
Organization name Berlin Institute of Health, Charité Medical University of Berlin
Department CUBI
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL15159
Series (2)
GSE70425 Cell-specific microRNA expression patterns in TB patients and household contacts
GSE70478 Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_25_P00013722 3.754956128
A_25_P00015543 3.224624214
A_25_P00013773 3.378427859
A_25_P00015229 3.688058354
A_25_P00016018 3.462317785
A_25_P00011991 6.151505537
A_25_P00015101 3.443362164
A_25_P00010432 5.009027235
A_25_P00015836 5.189493672
A_25_P00010259 3.281529656
A_25_P00015285 3.921704032
A_25_P00015097 3.549438689
A_25_P00012376 4.21318598
A_25_P00012139 4.315201925
A_25_P00015493 3.563003156
A_25_P00012297 3.457063155
A_25_P00010683 8.186039183
A_25_P00015031 3.470081233
A_25_P00015444 3.816288415
A_25_P00012542 3.764062682

Total number of rows: 3523

Table truncated, full table size 92 Kbytes.

Supplementary file Size Download File type/resource
GSM1754619_US22502595_253118112445_S01_miRNA_1100_Jul11_2_2.txt.gz 7.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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