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Sample GSM1717990 Query DataSets for GSM1717990
Status Public on Jan 15, 2016
Title MM034 control rep2
Sample type RNA
 
Source name short term culture
Organism Homo sapiens
Characteristics treatment: control
cell type: melanoma short term culture
Treatment protocol Cells were treated with a control gapmer or GapmeR3 targeting LINC01212. Alternatively, LINC01212 was overexpressed.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the miRNeasy mini kit (Qiagen)
Label Cy3
Label protocol Total RNA (100 ng) was labeled using the low input Quick Amp WT labeling kit (Agilent Technologies) according to the manufacturer’s instructions.
 
Hybridization protocol Labeled RNA was hybridized to a Agilent human gene expression microarray according to the manufacturer's instructions.
Scan protocol Arrays were scanned using the Agilent G2565CA Microarray Scanner and Scan Control Software 8.5 according to the manufacturer's instructions.
Data processing Probe intensities were extracted using Feature Extraction software (Agilent).
 
Submission date Jun 23, 2015
Last update date Jan 15, 2016
Contact name Pieter mestdagh
E-mail(s) pieter.mestdagh@ugent.be
Organization name Center for Medical Genetics
Street address De Pintelaan 185
City Gent
ZIP/Postal code 9000
Country Belgium
 
Platform ID GPL20604
Series (2)
GSE70176 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy [1]
GSE70180 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy

Data table header descriptions
ID_REF
VALUE Probe intensities were background corrected and normalized (quantile normalization) using the LIMMA package in R.

Data table
ID_REF VALUE
GE_BrightCorner 16.4698841
DarkCorner 3.959520271
A_33_P3400429 6.203143414
A_33_P3255531 5.115457029
A_24_P940149 4.662731032
A_33_P3306510 10.60529621
CUST_21085_PI427704219 4.32528684
A_33_P3348313 11.19254938
A_33_P3403361 11.71065339
A_33_P3348973 6.687761908
A_23_P11070 3.99472296
A_24_P274615 6.962932296
CUST_1480_PI427704219 5.078342838
CUST_1068_PI427704219 3.312944031
CUST_13414_PI427704219 4.609123289
A_24_P928969 7.429842318
A_23_P100074 12.8159457
A_33_P3227506 6.445876498
CUST_17237_PI427704219 6.380656561
CUST_12234_PI427704219 4.551441589

Total number of rows: 57244

Table truncated, full table size 1616 Kbytes.




Supplementary file Size Download File type/resource
GSM1717990_US45103088_254164810093_S01_GE1_105_Dec08_1_2.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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