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Sample GSM1717986 Query DataSets for GSM1717986
Status Public on Jan 15, 2016
Title SKMEL28 LINC01212 rep2
Sample type RNA
 
Source name cell line
Organism Homo sapiens
Characteristics treatment: LINC01212 overexpression
cell line: SKMEL28
Treatment protocol Cells were treated with a control gapmer or GapmeR3 targeting LINC01212. Alternatively, LINC01212 was overexpressed.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the miRNeasy mini kit (Qiagen)
Label Cy3
Label protocol Total RNA (100 ng) was labeled using the low input Quick Amp WT labeling kit (Agilent Technologies) according to the manufacturer’s instructions.
 
Hybridization protocol Labeled RNA was hybridized to a Agilent human gene expression microarray according to the manufacturer's instructions.
Scan protocol Arrays were scanned using the Agilent G2565CA Microarray Scanner and Scan Control Software 8.5 according to the manufacturer's instructions.
Data processing Probe intensities were extracted using Feature Extraction software (Agilent).
 
Submission date Jun 23, 2015
Last update date Jan 15, 2016
Contact name Pieter mestdagh
E-mail(s) pieter.mestdagh@ugent.be
Organization name Center for Medical Genetics
Street address De Pintelaan 185
City Gent
ZIP/Postal code 9000
Country Belgium
 
Platform ID GPL20604
Series (2)
GSE70176 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy [1]
GSE70180 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy

Data table header descriptions
ID_REF
VALUE Probe intensities were background corrected and normalized (quantile normalization) using the LIMMA package in R.

Data table
ID_REF VALUE
GE_BrightCorner 16.61830006
DarkCorner 3.622637166
A_33_P3400429 6.937698602
A_33_P3255531 7.201941567
A_24_P940149 6.34628029
A_33_P3306510 10.35890543
CUST_21085_PI427704219 4.312716231
A_33_P3348313 9.528035496
A_33_P3403361 10.88144475
A_33_P3348973 8.625879094
A_23_P11070 3.577851074
A_24_P274615 4.973935001
CUST_1480_PI427704219 6.322768514
CUST_1068_PI427704219 5.441604976
CUST_13414_PI427704219 5.160217254
A_24_P928969 7.953518858
A_23_P100074 9.765848668
A_33_P3227506 5.277454674
CUST_17237_PI427704219 5.863321373
CUST_12234_PI427704219 6.409444197

Total number of rows: 57244

Table truncated, full table size 1615 Kbytes.




Supplementary file Size Download File type/resource
GSM1717986_US45103088_254164810141_S01_GE1_105_Dec08_2_2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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