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Sample GSM1717985 Query DataSets for GSM1717985
Status Public on Jan 15, 2016
Title SKMEL28 LINC01212 rep1
Sample type RNA
 
Source name cell line
Organism Homo sapiens
Characteristics treatment: LINC01212 overexpression
cell line: SKMEL28
Treatment protocol Cells were treated with a control gapmer or GapmeR3 targeting LINC01212. Alternatively, LINC01212 was overexpressed.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the miRNeasy mini kit (Qiagen)
Label Cy3
Label protocol Total RNA (100 ng) was labeled using the low input Quick Amp WT labeling kit (Agilent Technologies) according to the manufacturer’s instructions.
 
Hybridization protocol Labeled RNA was hybridized to a Agilent human gene expression microarray according to the manufacturer's instructions.
Scan protocol Arrays were scanned using the Agilent G2565CA Microarray Scanner and Scan Control Software 8.5 according to the manufacturer's instructions.
Data processing Probe intensities were extracted using Feature Extraction software (Agilent).
 
Submission date Jun 23, 2015
Last update date Jan 15, 2016
Contact name Pieter mestdagh
E-mail(s) pieter.mestdagh@ugent.be
Organization name Center for Medical Genetics
Street address De Pintelaan 185
City Gent
ZIP/Postal code 9000
Country Belgium
 
Platform ID GPL20604
Series (2)
GSE70176 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy [1]
GSE70180 Targeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy

Data table header descriptions
ID_REF
VALUE Probe intensities were background corrected and normalized (quantile normalization) using the LIMMA package in R.

Data table
ID_REF VALUE
GE_BrightCorner 16.68109705
DarkCorner 3.633019311
A_33_P3400429 6.96224078
A_33_P3255531 4.78323383
A_24_P940149 6.525825249
A_33_P3306510 10.26323461
CUST_21085_PI427704219 5.576866715
A_33_P3348313 9.321579843
A_33_P3403361 10.80617561
A_33_P3348973 8.743491285
A_23_P11070 3.879657049
A_24_P274615 4.071032034
CUST_1480_PI427704219 4.128645494
CUST_1068_PI427704219 4.071032034
CUST_13414_PI427704219 5.25422295
A_24_P928969 8.257915008
A_23_P100074 9.888712213
A_33_P3227506 5.064819464
CUST_17237_PI427704219 5.397270491
CUST_12234_PI427704219 3.853961705

Total number of rows: 57244

Table truncated, full table size 1611 Kbytes.




Supplementary file Size Download File type/resource
GSM1717985_US45103088_254164810141_S01_GE1_105_Dec08_2_1.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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